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AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase

OBJECTIVE: Erectile dysfunction (ED) is one of the most common complications of diabetes mellitus (DM). Under the background that effectivity of treatments for diabetic ED are quite poor, stem cell therapy is emerging a useful method. The aim of this study is to evaluate the possibility and mechanis...

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Autores principales: Zhang, Yan, Wang, Tao, Yang, Jun, Li, Rui, Chen, Zhi, Wang, Shaogang, Liu, Jihong, Ye, Zhangqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708846/
http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s157
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author Zhang, Yan
Wang, Tao
Yang, Jun
Li, Rui
Chen, Zhi
Wang, Shaogang
Liu, Jihong
Ye, Zhangqun
author_facet Zhang, Yan
Wang, Tao
Yang, Jun
Li, Rui
Chen, Zhi
Wang, Shaogang
Liu, Jihong
Ye, Zhangqun
author_sort Zhang, Yan
collection PubMed
description OBJECTIVE: Erectile dysfunction (ED) is one of the most common complications of diabetes mellitus (DM). Under the background that effectivity of treatments for diabetic ED are quite poor, stem cell therapy is emerging a useful method. The aim of this study is to evaluate the possibility and mechanism of treatment of diabetic ED with endothelial progenitor cells (EPCs) genetically modified by human telomerase reverse transcriptase (hTERT). METHODS: Rat EPCs were isolated and transfected with hTERT (EPCs-hTERT). The paracrine characters and resistance to oxidative stress of EPCs-hTERT was determined in vitro. Thirty SD male rats were divided into five groups: diabetic ED rats, diabetic ED rats treated with EPCs, diabetic ED rats treated with EPCs-control, diabetic ED rats treated with EPCs-hTERT, normal control rats. Diabetes was induced by intraperitoneal injection of streptozotocin. After 8 weeks, diabetic ED rats were selected by apomorphine (APO) test. After injection of EPCs for 2 weeks, intracavernosal pressure (ICP) was measured by means of electrical stimulation for each group. Concentration of growth factors, the amount of EPCs and endothelium/smooth muscle content were evaluated in vivo. RESULTS: EPCs-hTERT showed stable expression of hTERT at the level of, mRNA and protein for more than 40 passages. EPCs-hTERT could paracrine more VEGF, HGF and bFGF. EPCs’ ability of resistance to oxidative stress was dramatically improved by tranfection of hTERT. ICP/mean arterial pressure (MAP) ratio induced by electrical stimulation in diabetic ED treated with EPCs-hTERT was markably higher than diabetic ED rats treated with EPCs or EPCs-control. Immunofluorescence demonstrated that more cells survived in penile tissues after implantation of EPC-hTERT. More growth factors were detected, and the endothelium/smooth muscle content was increase in penile tissues. CONCLUSIONS: The paracrine effect and resistance to oxidative stress of EPCs-hTERT contributed to the improvement of erection of diabetic ED rats.
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spelling pubmed-47088462016-01-26 AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase Zhang, Yan Wang, Tao Yang, Jun Li, Rui Chen, Zhi Wang, Shaogang Liu, Jihong Ye, Zhangqun Transl Androl Urol Moderated Poster Presentation OBJECTIVE: Erectile dysfunction (ED) is one of the most common complications of diabetes mellitus (DM). Under the background that effectivity of treatments for diabetic ED are quite poor, stem cell therapy is emerging a useful method. The aim of this study is to evaluate the possibility and mechanism of treatment of diabetic ED with endothelial progenitor cells (EPCs) genetically modified by human telomerase reverse transcriptase (hTERT). METHODS: Rat EPCs were isolated and transfected with hTERT (EPCs-hTERT). The paracrine characters and resistance to oxidative stress of EPCs-hTERT was determined in vitro. Thirty SD male rats were divided into five groups: diabetic ED rats, diabetic ED rats treated with EPCs, diabetic ED rats treated with EPCs-control, diabetic ED rats treated with EPCs-hTERT, normal control rats. Diabetes was induced by intraperitoneal injection of streptozotocin. After 8 weeks, diabetic ED rats were selected by apomorphine (APO) test. After injection of EPCs for 2 weeks, intracavernosal pressure (ICP) was measured by means of electrical stimulation for each group. Concentration of growth factors, the amount of EPCs and endothelium/smooth muscle content were evaluated in vivo. RESULTS: EPCs-hTERT showed stable expression of hTERT at the level of, mRNA and protein for more than 40 passages. EPCs-hTERT could paracrine more VEGF, HGF and bFGF. EPCs’ ability of resistance to oxidative stress was dramatically improved by tranfection of hTERT. ICP/mean arterial pressure (MAP) ratio induced by electrical stimulation in diabetic ED treated with EPCs-hTERT was markably higher than diabetic ED rats treated with EPCs or EPCs-control. Immunofluorescence demonstrated that more cells survived in penile tissues after implantation of EPC-hTERT. More growth factors were detected, and the endothelium/smooth muscle content was increase in penile tissues. CONCLUSIONS: The paracrine effect and resistance to oxidative stress of EPCs-hTERT contributed to the improvement of erection of diabetic ED rats. AME Publishing Company 2015-08 /pmc/articles/PMC4708846/ http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s157 Text en 2015 Translational Andrology and Urology. All rights reserved.
spellingShingle Moderated Poster Presentation
Zhang, Yan
Wang, Tao
Yang, Jun
Li, Rui
Chen, Zhi
Wang, Shaogang
Liu, Jihong
Ye, Zhangqun
AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title_full AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title_fullStr AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title_full_unstemmed AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title_short AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
title_sort ab157. treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
topic Moderated Poster Presentation
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4708846/
http://dx.doi.org/10.3978/j.issn.2223-4683.2015.s157
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