Benefits and Challenges with Applying Unique Molecular Identifiers in Next Generation Sequencing to Detect Low Frequency Mutations

Indexing individual template molecules with a unique identifier (UID) before PCR and deep sequencing is promising for detecting low frequency mutations, as true mutations could be distinguished from PCR errors or sequencing errors based on consensus among reads sharing same index. In an effort to de...

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Detalles Bibliográficos
Autores principales: Kou, Ruqin, Lam, Ham, Duan, Hairong, Ye, Li, Jongkam, Narisra, Chen, Weizhi, Zhang, Shifang, Li, Shihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709065/
https://www.ncbi.nlm.nih.gov/pubmed/26752634
http://dx.doi.org/10.1371/journal.pone.0146638

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