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Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking
Sensory cilia are populated by a select group of signaling proteins that detect environmental stimuli. How these molecules are delivered to the sensory cilium and whether they rely on one another for specific transport remains poorly understood. Here, we investigated whether the visual pigment, rhod...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709261/ https://www.ncbi.nlm.nih.gov/pubmed/26590321 http://dx.doi.org/10.7554/eLife.12058 |
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author | Pearring, Jillian N Spencer, William J Lieu, Eric C Arshavsky, Vadim Y |
author_facet | Pearring, Jillian N Spencer, William J Lieu, Eric C Arshavsky, Vadim Y |
author_sort | Pearring, Jillian N |
collection | PubMed |
description | Sensory cilia are populated by a select group of signaling proteins that detect environmental stimuli. How these molecules are delivered to the sensory cilium and whether they rely on one another for specific transport remains poorly understood. Here, we investigated whether the visual pigment, rhodopsin, is critical for delivering other signaling proteins to the sensory cilium of photoreceptor cells, the outer segment. Rhodopsin is the most abundant outer segment protein and its proper transport is essential for formation of this organelle, suggesting that such a dependency might exist. Indeed, we demonstrated that guanylate cyclase-1, producing the cGMP second messenger in photoreceptors, requires rhodopsin for intracellular stability and outer segment delivery. We elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein. These findings expand rhodopsin’s role in vision from being a visual pigment and major outer segment building block to directing trafficking of another key signaling protein. DOI: http://dx.doi.org/10.7554/eLife.12058.001 |
format | Online Article Text |
id | pubmed-4709261 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47092612016-01-13 Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking Pearring, Jillian N Spencer, William J Lieu, Eric C Arshavsky, Vadim Y eLife Neuroscience Sensory cilia are populated by a select group of signaling proteins that detect environmental stimuli. How these molecules are delivered to the sensory cilium and whether they rely on one another for specific transport remains poorly understood. Here, we investigated whether the visual pigment, rhodopsin, is critical for delivering other signaling proteins to the sensory cilium of photoreceptor cells, the outer segment. Rhodopsin is the most abundant outer segment protein and its proper transport is essential for formation of this organelle, suggesting that such a dependency might exist. Indeed, we demonstrated that guanylate cyclase-1, producing the cGMP second messenger in photoreceptors, requires rhodopsin for intracellular stability and outer segment delivery. We elucidated this dependency by showing that guanylate cyclase-1 is a novel rhodopsin-binding protein. These findings expand rhodopsin’s role in vision from being a visual pigment and major outer segment building block to directing trafficking of another key signaling protein. DOI: http://dx.doi.org/10.7554/eLife.12058.001 eLife Sciences Publications, Ltd 2015-11-21 /pmc/articles/PMC4709261/ /pubmed/26590321 http://dx.doi.org/10.7554/eLife.12058 Text en © 2015, Pearring et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Pearring, Jillian N Spencer, William J Lieu, Eric C Arshavsky, Vadim Y Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title | Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title_full | Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title_fullStr | Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title_full_unstemmed | Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title_short | Guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
title_sort | guanylate cyclase 1 relies on rhodopsin for intracellular stability and ciliary trafficking |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709261/ https://www.ncbi.nlm.nih.gov/pubmed/26590321 http://dx.doi.org/10.7554/eLife.12058 |
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