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Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue
E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mous...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709331/ https://www.ncbi.nlm.nih.gov/pubmed/26725115 http://dx.doi.org/10.1016/j.celrep.2015.12.020 |
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author | Erami, Zahra Herrmann, David Warren, Sean C. Nobis, Max McGhee, Ewan J. Lucas, Morghan C. Leung, Wilfred Reischmann, Nadine Mrowinska, Agata Schwarz, Juliane P. Kadir, Shereen Conway, James R.W. Vennin, Claire Karim, Saadia A. Campbell, Andrew D. Gallego-Ortega, David Magenau, Astrid Murphy, Kendelle J. Ridgway, Rachel A. Law, Andrew M. Walters, Stacey N. Grey, Shane T. Croucher, David R. Zhang, Lei Herzog, Herbert Hardeman, Edna C. Gunning, Peter W. Ormandy, Christopher J. Evans, T.R. Jeffry Strathdee, Douglas Sansom, Owen J. Morton, Jennifer P. Anderson, Kurt I. Timpson, Paul |
author_facet | Erami, Zahra Herrmann, David Warren, Sean C. Nobis, Max McGhee, Ewan J. Lucas, Morghan C. Leung, Wilfred Reischmann, Nadine Mrowinska, Agata Schwarz, Juliane P. Kadir, Shereen Conway, James R.W. Vennin, Claire Karim, Saadia A. Campbell, Andrew D. Gallego-Ortega, David Magenau, Astrid Murphy, Kendelle J. Ridgway, Rachel A. Law, Andrew M. Walters, Stacey N. Grey, Shane T. Croucher, David R. Zhang, Lei Herzog, Herbert Hardeman, Edna C. Gunning, Peter W. Ormandy, Christopher J. Evans, T.R. Jeffry Strathdee, Douglas Sansom, Owen J. Morton, Jennifer P. Anderson, Kurt I. Timpson, Paul |
author_sort | Erami, Zahra |
collection | PubMed |
description | E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments. |
format | Online Article Text |
id | pubmed-4709331 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-47093312016-02-10 Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue Erami, Zahra Herrmann, David Warren, Sean C. Nobis, Max McGhee, Ewan J. Lucas, Morghan C. Leung, Wilfred Reischmann, Nadine Mrowinska, Agata Schwarz, Juliane P. Kadir, Shereen Conway, James R.W. Vennin, Claire Karim, Saadia A. Campbell, Andrew D. Gallego-Ortega, David Magenau, Astrid Murphy, Kendelle J. Ridgway, Rachel A. Law, Andrew M. Walters, Stacey N. Grey, Shane T. Croucher, David R. Zhang, Lei Herzog, Herbert Hardeman, Edna C. Gunning, Peter W. Ormandy, Christopher J. Evans, T.R. Jeffry Strathdee, Douglas Sansom, Owen J. Morton, Jennifer P. Anderson, Kurt I. Timpson, Paul Cell Rep Resource E-cadherin-mediated cell-cell junctions play a prominent role in maintaining the epithelial architecture. The disruption or deregulation of these adhesions in cancer can lead to the collapse of tumor epithelia that precedes invasion and subsequent metastasis. Here we generated an E-cadherin-GFP mouse that enables intravital photobleaching and quantification of E-cadherin mobility in live tissue without affecting normal biology. We demonstrate the broad applications of this mouse by examining E-cadherin regulation in multiple tissues, including mammary, brain, liver, and kidney tissue, while specifically monitoring E-cadherin mobility during disease progression in the pancreas. We assess E-cadherin stability in native pancreatic tissue upon genetic manipulation involving Kras and p53 or in response to anti-invasive drug treatment and gain insights into the dynamic remodeling of E-cadherin during in situ cancer progression. FRAP in the E-cadherin-GFP mouse, therefore, promises to be a valuable tool to fundamentally expand our understanding of E-cadherin-mediated events in native microenvironments. Cell Press 2015-12-24 /pmc/articles/PMC4709331/ /pubmed/26725115 http://dx.doi.org/10.1016/j.celrep.2015.12.020 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Resource Erami, Zahra Herrmann, David Warren, Sean C. Nobis, Max McGhee, Ewan J. Lucas, Morghan C. Leung, Wilfred Reischmann, Nadine Mrowinska, Agata Schwarz, Juliane P. Kadir, Shereen Conway, James R.W. Vennin, Claire Karim, Saadia A. Campbell, Andrew D. Gallego-Ortega, David Magenau, Astrid Murphy, Kendelle J. Ridgway, Rachel A. Law, Andrew M. Walters, Stacey N. Grey, Shane T. Croucher, David R. Zhang, Lei Herzog, Herbert Hardeman, Edna C. Gunning, Peter W. Ormandy, Christopher J. Evans, T.R. Jeffry Strathdee, Douglas Sansom, Owen J. Morton, Jennifer P. Anderson, Kurt I. Timpson, Paul Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title | Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title_full | Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title_fullStr | Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title_full_unstemmed | Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title_short | Intravital FRAP Imaging using an E-cadherin-GFP Mouse Reveals Disease- and Drug-Dependent Dynamic Regulation of Cell-Cell Junctions in Live Tissue |
title_sort | intravital frap imaging using an e-cadherin-gfp mouse reveals disease- and drug-dependent dynamic regulation of cell-cell junctions in live tissue |
topic | Resource |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709331/ https://www.ncbi.nlm.nih.gov/pubmed/26725115 http://dx.doi.org/10.1016/j.celrep.2015.12.020 |
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