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Optimising methods for the preservation, capture and identification of ubiquitin chains and ubiquitylated proteins by immunoblotting

Immunoblotting is a powerful technique for the semi-quantitative analysis of ubiquitylation events, and remains the most commonly used method to study this process due to its high specificity, speed, sensitivity and relatively low cost. However, the ubiquitylation of proteins is complex and, when th...

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Detalles Bibliográficos
Autores principales: Emmerich, Christoph H., Cohen, Philip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709362/
https://www.ncbi.nlm.nih.gov/pubmed/26325464
http://dx.doi.org/10.1016/j.bbrc.2015.08.109