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TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling

Tripartite Motif Containing 25 (TRIM25), a member of TRIM proteins, has been found abnormally expressed in cancers of female reproductive system. Here, TRIM25 was conspicuously expressed in human gastric cancer (GC) tissues in which its higher expression generally correlated with the poor prognosis...

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Autores principales: Zhu, Zhenya, Wang, Yong, Zhang, Chunhui, Yu, Shiyong, Zhu, Qi, Hou, Kun, Yan, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709557/
https://www.ncbi.nlm.nih.gov/pubmed/26754079
http://dx.doi.org/10.1038/srep19070
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author Zhu, Zhenya
Wang, Yong
Zhang, Chunhui
Yu, Shiyong
Zhu, Qi
Hou, Kun
Yan, Bo
author_facet Zhu, Zhenya
Wang, Yong
Zhang, Chunhui
Yu, Shiyong
Zhu, Qi
Hou, Kun
Yan, Bo
author_sort Zhu, Zhenya
collection PubMed
description Tripartite Motif Containing 25 (TRIM25), a member of TRIM proteins, has been found abnormally expressed in cancers of female reproductive system. Here, TRIM25 was conspicuously expressed in human gastric cancer (GC) tissues in which its higher expression generally correlated with the poor prognosis of patients. Small interfering RNA (siRNA)-mediated knockdown of TRIM25 expression in MGC-803 and AGS cells had no effects on cell proliferation, whereas reduced cell migration and invasion. Gene set enrichment analysis on The Cancer Genome Atlas stomach adenocarcinoma (STAD) dataset revealed that several signaling pathways, including the migration, E-cadherin and transforming growth factor-β (TGF-β) pathways, were enriched in TRIM25 higher expression patients. Moreover, ectopic expression of TRIM25 in a GC cell line with lower expression of TRIM25 significantly promoted the migration and invasion. Further experiments with TGF-β inhibitor suggested that TRIM25 may exert its function through TGF-β pathway. In summary, our results indicate that TRIM25 acts as an oncogene in GC and thus presents a novel target for the detection and treatment of GC.
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spelling pubmed-47095572016-01-20 TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling Zhu, Zhenya Wang, Yong Zhang, Chunhui Yu, Shiyong Zhu, Qi Hou, Kun Yan, Bo Sci Rep Article Tripartite Motif Containing 25 (TRIM25), a member of TRIM proteins, has been found abnormally expressed in cancers of female reproductive system. Here, TRIM25 was conspicuously expressed in human gastric cancer (GC) tissues in which its higher expression generally correlated with the poor prognosis of patients. Small interfering RNA (siRNA)-mediated knockdown of TRIM25 expression in MGC-803 and AGS cells had no effects on cell proliferation, whereas reduced cell migration and invasion. Gene set enrichment analysis on The Cancer Genome Atlas stomach adenocarcinoma (STAD) dataset revealed that several signaling pathways, including the migration, E-cadherin and transforming growth factor-β (TGF-β) pathways, were enriched in TRIM25 higher expression patients. Moreover, ectopic expression of TRIM25 in a GC cell line with lower expression of TRIM25 significantly promoted the migration and invasion. Further experiments with TGF-β inhibitor suggested that TRIM25 may exert its function through TGF-β pathway. In summary, our results indicate that TRIM25 acts as an oncogene in GC and thus presents a novel target for the detection and treatment of GC. Nature Publishing Group 2016-01-12 /pmc/articles/PMC4709557/ /pubmed/26754079 http://dx.doi.org/10.1038/srep19070 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Zhu, Zhenya
Wang, Yong
Zhang, Chunhui
Yu, Shiyong
Zhu, Qi
Hou, Kun
Yan, Bo
TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title_full TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title_fullStr TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title_full_unstemmed TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title_short TRIM25 blockade by RNA interference inhibited migration and invasion of gastric cancer cells through TGF-β signaling
title_sort trim25 blockade by rna interference inhibited migration and invasion of gastric cancer cells through tgf-β signaling
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709557/
https://www.ncbi.nlm.nih.gov/pubmed/26754079
http://dx.doi.org/10.1038/srep19070
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