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APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea

Lepidopteran midgut aminopeptidases N (APNs) are phylogenetically divided into eight clusters, designated as APN1–8. Although APN1 has been implicated as one of the receptors for Cry1Ac in several species, its potential role in the mode of action of Cry2Ab has not been functionally determined so far...

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Autores principales: Wei, Jizhen, Zhang, Min, Liang, Gemei, Wu, Kongming, Guo, Yuyuan, Ni, Xinzhi, Li, Xianchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709634/
https://www.ncbi.nlm.nih.gov/pubmed/26755166
http://dx.doi.org/10.1038/srep19179
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author Wei, Jizhen
Zhang, Min
Liang, Gemei
Wu, Kongming
Guo, Yuyuan
Ni, Xinzhi
Li, Xianchun
author_facet Wei, Jizhen
Zhang, Min
Liang, Gemei
Wu, Kongming
Guo, Yuyuan
Ni, Xinzhi
Li, Xianchun
author_sort Wei, Jizhen
collection PubMed
description Lepidopteran midgut aminopeptidases N (APNs) are phylogenetically divided into eight clusters, designated as APN1–8. Although APN1 has been implicated as one of the receptors for Cry1Ac in several species, its potential role in the mode of action of Cry2Ab has not been functionally determined so far. To test whether APN1 also acts as one of the receptors for Cry1Ac in Helicoverpa zea and even for Cry2Ab in this species, we conducted a gain of function analysis by heterologously expressing H. zea APN1 (HzAPN1) in the midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) and a loss of function analysis by RNAi (RNA interference) silencing of the endogenous APN1 in the three cell lines using the HzAPN1 double strand RNA (dsRNA). Heterologous expression of HzAPN1 significantly increased the susceptibility of the three cell lines to Cry1Ac, but had no effects on their susceptibility to Cry2Ab. Knocking down of the endogenous APN1 made the three cell lines resistant to Cry1Ac, but didn’t change cell lines susceptibility to Cry2Ab. The findings from this study demonstrate that HzAPN1 is a functional receptor of Cry1Ac, but not Cry2Ab.
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spelling pubmed-47096342016-01-20 APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea Wei, Jizhen Zhang, Min Liang, Gemei Wu, Kongming Guo, Yuyuan Ni, Xinzhi Li, Xianchun Sci Rep Article Lepidopteran midgut aminopeptidases N (APNs) are phylogenetically divided into eight clusters, designated as APN1–8. Although APN1 has been implicated as one of the receptors for Cry1Ac in several species, its potential role in the mode of action of Cry2Ab has not been functionally determined so far. To test whether APN1 also acts as one of the receptors for Cry1Ac in Helicoverpa zea and even for Cry2Ab in this species, we conducted a gain of function analysis by heterologously expressing H. zea APN1 (HzAPN1) in the midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) and a loss of function analysis by RNAi (RNA interference) silencing of the endogenous APN1 in the three cell lines using the HzAPN1 double strand RNA (dsRNA). Heterologous expression of HzAPN1 significantly increased the susceptibility of the three cell lines to Cry1Ac, but had no effects on their susceptibility to Cry2Ab. Knocking down of the endogenous APN1 made the three cell lines resistant to Cry1Ac, but didn’t change cell lines susceptibility to Cry2Ab. The findings from this study demonstrate that HzAPN1 is a functional receptor of Cry1Ac, but not Cry2Ab. Nature Publishing Group 2016-01-12 /pmc/articles/PMC4709634/ /pubmed/26755166 http://dx.doi.org/10.1038/srep19179 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wei, Jizhen
Zhang, Min
Liang, Gemei
Wu, Kongming
Guo, Yuyuan
Ni, Xinzhi
Li, Xianchun
APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title_full APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title_fullStr APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title_full_unstemmed APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title_short APN1 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea
title_sort apn1 is a functional receptor of cry1ac but not cry2ab in helicoverpa zea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709634/
https://www.ncbi.nlm.nih.gov/pubmed/26755166
http://dx.doi.org/10.1038/srep19179
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