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In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy
Ionizing radiation (IR) injury is tissue damage caused by high energy electromagnetic waves such as X-ray and gamma ray. Diagnosis and treatment of IR injury are difficult due to its characteristics of clinically latent post-irradiation periods and the following successive and unpredictable inflamma...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709756/ https://www.ncbi.nlm.nih.gov/pubmed/26755422 http://dx.doi.org/10.1038/srep19216 |
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author | Jang, Won Hyuk Shim, Sehwan Wang, Taejun Yoon, Yeoreum Jang, Won-Suk Myung, Jae Kyung Park, Sunhoo Kim, Ki Hean |
author_facet | Jang, Won Hyuk Shim, Sehwan Wang, Taejun Yoon, Yeoreum Jang, Won-Suk Myung, Jae Kyung Park, Sunhoo Kim, Ki Hean |
author_sort | Jang, Won Hyuk |
collection | PubMed |
description | Ionizing radiation (IR) injury is tissue damage caused by high energy electromagnetic waves such as X-ray and gamma ray. Diagnosis and treatment of IR injury are difficult due to its characteristics of clinically latent post-irradiation periods and the following successive and unpredictable inflammatory bursts. Skin is one of the many sensitive organs to IR and bears local injury upon exposure. Early-stage diagnosis of IR skin injury is essential in order to maximize treatment efficiency and to prevent the aggravation of IR injury. In this study, early-stage changes of the IR injured skin at the cellular level were characterized in an in vivo mouse model by two-photon microscopy (TPM). Various IR doses were applied to the mouse hind limbs and the injured skin regions were imaged daily for 6 days after IR irradiation. Changes in the morphology and distribution of the epidermal cells and damage of the sebaceous glands were observed before clinical symptoms. These results showed that TPM is sensitive to early-stage changes of IR skin injury and may be useful for its diagnosis. |
format | Online Article Text |
id | pubmed-4709756 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-47097562016-01-20 In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy Jang, Won Hyuk Shim, Sehwan Wang, Taejun Yoon, Yeoreum Jang, Won-Suk Myung, Jae Kyung Park, Sunhoo Kim, Ki Hean Sci Rep Article Ionizing radiation (IR) injury is tissue damage caused by high energy electromagnetic waves such as X-ray and gamma ray. Diagnosis and treatment of IR injury are difficult due to its characteristics of clinically latent post-irradiation periods and the following successive and unpredictable inflammatory bursts. Skin is one of the many sensitive organs to IR and bears local injury upon exposure. Early-stage diagnosis of IR skin injury is essential in order to maximize treatment efficiency and to prevent the aggravation of IR injury. In this study, early-stage changes of the IR injured skin at the cellular level were characterized in an in vivo mouse model by two-photon microscopy (TPM). Various IR doses were applied to the mouse hind limbs and the injured skin regions were imaged daily for 6 days after IR irradiation. Changes in the morphology and distribution of the epidermal cells and damage of the sebaceous glands were observed before clinical symptoms. These results showed that TPM is sensitive to early-stage changes of IR skin injury and may be useful for its diagnosis. Nature Publishing Group 2016-01-12 /pmc/articles/PMC4709756/ /pubmed/26755422 http://dx.doi.org/10.1038/srep19216 Text en Copyright © 2016, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Jang, Won Hyuk Shim, Sehwan Wang, Taejun Yoon, Yeoreum Jang, Won-Suk Myung, Jae Kyung Park, Sunhoo Kim, Ki Hean In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title | In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title_full | In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title_fullStr | In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title_full_unstemmed | In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title_short | In vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
title_sort | in vivo characterization of early-stage radiation skin injury in a mouse model by two-photon microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4709756/ https://www.ncbi.nlm.nih.gov/pubmed/26755422 http://dx.doi.org/10.1038/srep19216 |
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