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Direct next-generation sequencing of virus-human mixed samples without pretreatment is favorable to recover virus genome

ABSTRACT: Next-generation sequencing (NGS) enables the recovery of pathogen genomes from clinical samples without the need for culturing. Depletion of host/microbiota components (e.g., ribosomal RNA and poly-A RNA) and whole DNA/cDNA amplification are routine methods to improve recovery results. Usi...

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Detalles Bibliográficos
Autores principales:	Li, Dingchen, Li, Zongwei, Zhou, Zhe, Li, Zhen, Qu, Xinyan, Xu, Peisong, Zhou, Pingkun, Bo, Xiaochen, Ni, Ming
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2016
Materias:	Discovery Notes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710016/ https://www.ncbi.nlm.nih.gov/pubmed/26754142 http://dx.doi.org/10.1186/s13062-016-0105-x

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