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A comprehensive model to predict mitotic division in budding yeasts
High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomy...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710229/ https://www.ncbi.nlm.nih.gov/pubmed/26310442 http://dx.doi.org/10.1091/mbc.E15-04-0236 |
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author | Sutradhar, Sabyasachi Yadav, Vikas Sridhar, Shreyas Sreekumar, Lakshmi Bhattacharyya, Dibyendu Ghosh, Santanu Kumar Paul, Raja Sanyal, Kaustuv |
author_facet | Sutradhar, Sabyasachi Yadav, Vikas Sridhar, Shreyas Sreekumar, Lakshmi Bhattacharyya, Dibyendu Ghosh, Santanu Kumar Paul, Raja Sanyal, Kaustuv |
author_sort | Sutradhar, Sabyasachi |
collection | PubMed |
description | High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division. |
format | Online Article Text |
id | pubmed-4710229 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-47102292016-01-20 A comprehensive model to predict mitotic division in budding yeasts Sutradhar, Sabyasachi Yadav, Vikas Sridhar, Shreyas Sreekumar, Lakshmi Bhattacharyya, Dibyendu Ghosh, Santanu Kumar Paul, Raja Sanyal, Kaustuv Mol Biol Cell Articles High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division. The American Society for Cell Biology 2015-11-05 /pmc/articles/PMC4710229/ /pubmed/26310442 http://dx.doi.org/10.1091/mbc.E15-04-0236 Text en © 2015 Sutradhar, Yadav, Sridhar, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Sutradhar, Sabyasachi Yadav, Vikas Sridhar, Shreyas Sreekumar, Lakshmi Bhattacharyya, Dibyendu Ghosh, Santanu Kumar Paul, Raja Sanyal, Kaustuv A comprehensive model to predict mitotic division in budding yeasts |
title | A comprehensive model to predict mitotic division in budding yeasts |
title_full | A comprehensive model to predict mitotic division in budding yeasts |
title_fullStr | A comprehensive model to predict mitotic division in budding yeasts |
title_full_unstemmed | A comprehensive model to predict mitotic division in budding yeasts |
title_short | A comprehensive model to predict mitotic division in budding yeasts |
title_sort | comprehensive model to predict mitotic division in budding yeasts |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710229/ https://www.ncbi.nlm.nih.gov/pubmed/26310442 http://dx.doi.org/10.1091/mbc.E15-04-0236 |
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