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A comprehensive model to predict mitotic division in budding yeasts

High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomy...

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Autores principales: Sutradhar, Sabyasachi, Yadav, Vikas, Sridhar, Shreyas, Sreekumar, Lakshmi, Bhattacharyya, Dibyendu, Ghosh, Santanu Kumar, Paul, Raja, Sanyal, Kaustuv
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710229/
https://www.ncbi.nlm.nih.gov/pubmed/26310442
http://dx.doi.org/10.1091/mbc.E15-04-0236
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author Sutradhar, Sabyasachi
Yadav, Vikas
Sridhar, Shreyas
Sreekumar, Lakshmi
Bhattacharyya, Dibyendu
Ghosh, Santanu Kumar
Paul, Raja
Sanyal, Kaustuv
author_facet Sutradhar, Sabyasachi
Yadav, Vikas
Sridhar, Shreyas
Sreekumar, Lakshmi
Bhattacharyya, Dibyendu
Ghosh, Santanu Kumar
Paul, Raja
Sanyal, Kaustuv
author_sort Sutradhar, Sabyasachi
collection PubMed
description High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division.
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spelling pubmed-47102292016-01-20 A comprehensive model to predict mitotic division in budding yeasts Sutradhar, Sabyasachi Yadav, Vikas Sridhar, Shreyas Sreekumar, Lakshmi Bhattacharyya, Dibyendu Ghosh, Santanu Kumar Paul, Raja Sanyal, Kaustuv Mol Biol Cell Articles High-fidelity chromosome segregation during cell division depends on a series of concerted interdependent interactions. Using a systems biology approach, we built a robust minimal computational model to comprehend mitotic events in dividing budding yeasts of two major phyla: Ascomycota and Basidiomycota. This model accurately reproduces experimental observations related to spindle alignment, nuclear migration, and microtubule (MT) dynamics during cell division in these yeasts. The model converges to the conclusion that biased nucleation of cytoplasmic microtubules (cMTs) is essential for directional nuclear migration. Two distinct pathways, based on the population of cMTs and cortical dyneins, differentiate nuclear migration and spindle orientation in these two phyla. In addition, the model accurately predicts the contribution of specific classes of MTs in chromosome segregation. Thus we present a model that offers a wider applicability to simulate the effects of perturbation of an event on the concerted process of the mitotic cell division. The American Society for Cell Biology 2015-11-05 /pmc/articles/PMC4710229/ /pubmed/26310442 http://dx.doi.org/10.1091/mbc.E15-04-0236 Text en © 2015 Sutradhar, Yadav, Sridhar, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Sutradhar, Sabyasachi
Yadav, Vikas
Sridhar, Shreyas
Sreekumar, Lakshmi
Bhattacharyya, Dibyendu
Ghosh, Santanu Kumar
Paul, Raja
Sanyal, Kaustuv
A comprehensive model to predict mitotic division in budding yeasts
title A comprehensive model to predict mitotic division in budding yeasts
title_full A comprehensive model to predict mitotic division in budding yeasts
title_fullStr A comprehensive model to predict mitotic division in budding yeasts
title_full_unstemmed A comprehensive model to predict mitotic division in budding yeasts
title_short A comprehensive model to predict mitotic division in budding yeasts
title_sort comprehensive model to predict mitotic division in budding yeasts
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710229/
https://www.ncbi.nlm.nih.gov/pubmed/26310442
http://dx.doi.org/10.1091/mbc.E15-04-0236
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