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Optimized Negative Staining: a High-throughput Protocol for Examining Small and Asymmetric Protein Structure by Electron Microscopy

Structural determination of proteins is rather challenging for proteins with molecular masses between 40 - 200 kDa. Considering that more than half of natural proteins have a molecular mass between 40 - 200 kDa(1,2), a robust and high-throughput method with a nanometer resolution capability is neede...

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Detalles Bibliográficos
Autores principales: Rames, Matthew, Yu, Yadong, Ren, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710468/
https://www.ncbi.nlm.nih.gov/pubmed/25145703
http://dx.doi.org/10.3791/51087

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