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Whole-brain activity mapping onto a zebrafish brain atlas
In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open source atlas containing molecular labels and anatom...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710481/ https://www.ncbi.nlm.nih.gov/pubmed/26778924 http://dx.doi.org/10.1038/nmeth.3581 |
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author | Randlett, Owen Wee, Caroline L. Naumann, Eva A. Nnaemeka, Onyeka Schoppik, David Fitzgerald, James E. Portugues, Ruben Lacoste, Alix M.B. Riegler, Clemens Engert, Florian Schier, Alexander F. |
author_facet | Randlett, Owen Wee, Caroline L. Naumann, Eva A. Nnaemeka, Onyeka Schoppik, David Fitzgerald, James E. Portugues, Ruben Lacoste, Alix M.B. Riegler, Clemens Engert, Florian Schier, Alexander F. |
author_sort | Randlett, Owen |
collection | PubMed |
description | In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open source atlas containing molecular labels and anatomical region definitions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated-Extracellular signal-regulated kinase (ERK/MAPK) as a readout of neural activity, we have developed a system to create and contextualize whole brain maps of stimulus- and behavior-dependent neural activity. This MAP-Mapping (Mitogen Activated Protein kinase – Mapping) assay is technically simple, fast, inexpensive, and data analysis is completely automated. Since MAP-Mapping is performed on fish that are freely swimming, it is applicable to nearly any stimulus or behavior. We demonstrate the utility of our high-throughput approach using hunting/feeding, pharmacological, visual and noxious stimuli. The resultant maps outline hundreds of areas associated with behaviors. |
format | Online Article Text |
id | pubmed-4710481 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-47104812016-05-01 Whole-brain activity mapping onto a zebrafish brain atlas Randlett, Owen Wee, Caroline L. Naumann, Eva A. Nnaemeka, Onyeka Schoppik, David Fitzgerald, James E. Portugues, Ruben Lacoste, Alix M.B. Riegler, Clemens Engert, Florian Schier, Alexander F. Nat Methods Article In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open source atlas containing molecular labels and anatomical region definitions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated-Extracellular signal-regulated kinase (ERK/MAPK) as a readout of neural activity, we have developed a system to create and contextualize whole brain maps of stimulus- and behavior-dependent neural activity. This MAP-Mapping (Mitogen Activated Protein kinase – Mapping) assay is technically simple, fast, inexpensive, and data analysis is completely automated. Since MAP-Mapping is performed on fish that are freely swimming, it is applicable to nearly any stimulus or behavior. We demonstrate the utility of our high-throughput approach using hunting/feeding, pharmacological, visual and noxious stimuli. The resultant maps outline hundreds of areas associated with behaviors. 2015-09-14 2015-11 /pmc/articles/PMC4710481/ /pubmed/26778924 http://dx.doi.org/10.1038/nmeth.3581 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Randlett, Owen Wee, Caroline L. Naumann, Eva A. Nnaemeka, Onyeka Schoppik, David Fitzgerald, James E. Portugues, Ruben Lacoste, Alix M.B. Riegler, Clemens Engert, Florian Schier, Alexander F. Whole-brain activity mapping onto a zebrafish brain atlas |
title | Whole-brain activity mapping onto a zebrafish brain atlas |
title_full | Whole-brain activity mapping onto a zebrafish brain atlas |
title_fullStr | Whole-brain activity mapping onto a zebrafish brain atlas |
title_full_unstemmed | Whole-brain activity mapping onto a zebrafish brain atlas |
title_short | Whole-brain activity mapping onto a zebrafish brain atlas |
title_sort | whole-brain activity mapping onto a zebrafish brain atlas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710481/ https://www.ncbi.nlm.nih.gov/pubmed/26778924 http://dx.doi.org/10.1038/nmeth.3581 |
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