Quantitative PCR detection of feline morbillivirus in cat urine samples

Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence...

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Autores principales: FURUYA, Tetsuya, WACHI, Akiko, SASSA, Yukiko, OMATSU, Tsutomu, NAGAI, Makoto, FUKUSHIMA, Ryuji, SHIBUTANI, Makoto, YAMAGUCHI, Tomohiro, UEMATSU, Yosuke, SHIROTA, Kinji, MIZUTANI, Tetsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2015
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710735/
https://www.ncbi.nlm.nih.gov/pubmed/26227478
http://dx.doi.org/10.1292/jvms.15-0112
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author FURUYA, Tetsuya
WACHI, Akiko
SASSA, Yukiko
OMATSU, Tsutomu
NAGAI, Makoto
FUKUSHIMA, Ryuji
SHIBUTANI, Makoto
YAMAGUCHI, Tomohiro
UEMATSU, Yosuke
SHIROTA, Kinji
MIZUTANI, Tetsuya
author_facet FURUYA, Tetsuya
WACHI, Akiko
SASSA, Yukiko
OMATSU, Tsutomu
NAGAI, Makoto
FUKUSHIMA, Ryuji
SHIBUTANI, Makoto
YAMAGUCHI, Tomohiro
UEMATSU, Yosuke
SHIROTA, Kinji
MIZUTANI, Tetsuya
author_sort FURUYA, Tetsuya
collection PubMed
description Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples.
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spelling pubmed-47107352016-01-14 Quantitative PCR detection of feline morbillivirus in cat urine samples FURUYA, Tetsuya WACHI, Akiko SASSA, Yukiko OMATSU, Tsutomu NAGAI, Makoto FUKUSHIMA, Ryuji SHIBUTANI, Makoto YAMAGUCHI, Tomohiro UEMATSU, Yosuke SHIROTA, Kinji MIZUTANI, Tetsuya J Vet Med Sci Virology Feline morbillivirus (FmoPV) is a new virus species and its detection is important, since correlation has been reported between FmoPV virus infection and tubulointerstitial nephritis in cats. Here, we report a real-time reverse transcription (RT)-PCR system that can detect the FmoPV L-gene sequence with more than 10-time higher sensitivity than a conventional PCR system, resulting in detection of less than 10 copies of the template DNA. The total FmoPV positive rate of urine samples from veterinary clinics and hospitals in Japan was 15.1% (25/166) using this system. This study demonstrates usefulness of the real-time RT-PCR system for detection of FmoPV for cat urine samples. The Japanese Society of Veterinary Science 2015-07-26 2015-12 /pmc/articles/PMC4710735/ /pubmed/26227478 http://dx.doi.org/10.1292/jvms.15-0112 Text en ©2015 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Virology
FURUYA, Tetsuya
WACHI, Akiko
SASSA, Yukiko
OMATSU, Tsutomu
NAGAI, Makoto
FUKUSHIMA, Ryuji
SHIBUTANI, Makoto
YAMAGUCHI, Tomohiro
UEMATSU, Yosuke
SHIROTA, Kinji
MIZUTANI, Tetsuya
Quantitative PCR detection of feline morbillivirus in cat urine samples
title Quantitative PCR detection of feline morbillivirus in cat urine samples
title_full Quantitative PCR detection of feline morbillivirus in cat urine samples
title_fullStr Quantitative PCR detection of feline morbillivirus in cat urine samples
title_full_unstemmed Quantitative PCR detection of feline morbillivirus in cat urine samples
title_short Quantitative PCR detection of feline morbillivirus in cat urine samples
title_sort quantitative pcr detection of feline morbillivirus in cat urine samples
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4710735/
https://www.ncbi.nlm.nih.gov/pubmed/26227478
http://dx.doi.org/10.1292/jvms.15-0112
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