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Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma

Colorectal cancer (CRC) is the third most common malignancy and the second most common cause of cancer death worldwide. Early detection of CRC can improve patient survival rates; thus, the identification of noninvasive diagnostic markers is urgently needed. MicroRNAs (miRNAs) have extensive potentia...

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Autores principales: Ghanbari, Reza, Mosakhani, Neda, Sarhadi, Virinder K., Armengol, Gemma, Nouraee, Nazila, Mohammadkhani, Ashraf, Khorrami, Samaneh, Arefian, Ehsan, Paryan, Mahdi, Malekzadeh, Reza, Knuutila, Sakari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Libertas Academica 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711391/
https://www.ncbi.nlm.nih.gov/pubmed/26793011
http://dx.doi.org/10.4137/BIC.S25252
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author Ghanbari, Reza
Mosakhani, Neda
Sarhadi, Virinder K.
Armengol, Gemma
Nouraee, Nazila
Mohammadkhani, Ashraf
Khorrami, Samaneh
Arefian, Ehsan
Paryan, Mahdi
Malekzadeh, Reza
Knuutila, Sakari
author_facet Ghanbari, Reza
Mosakhani, Neda
Sarhadi, Virinder K.
Armengol, Gemma
Nouraee, Nazila
Mohammadkhani, Ashraf
Khorrami, Samaneh
Arefian, Ehsan
Paryan, Mahdi
Malekzadeh, Reza
Knuutila, Sakari
author_sort Ghanbari, Reza
collection PubMed
description Colorectal cancer (CRC) is the third most common malignancy and the second most common cause of cancer death worldwide. Early detection of CRC can improve patient survival rates; thus, the identification of noninvasive diagnostic markers is urgently needed. MicroRNAs (miRNAs) have extensive potential to diagnose several diseases, including cancer. In this study, we compared the expression pattern of miRNAs from plasma and stool samples of patients with early stages of CRC (I, II) with that of healthy subjects. We performed miRNA profiling using microarrays on plasma and stool samples of eight patients with CRC and four healthy subjects. Seven miRNAs were found to be underexpressed in both plasma and stool samples of patients with CRC versus healthy subjects. Then, we aimed to verify two out of these seven differentially expressed miRNAs (let-7a-5p and let-7f-5p) by quantitative reverse transcriptase polymerase chain reaction on a larger set of plasma and stool samples of 51 patients with CRC and 26 healthy subjects. We confirmed the results of microarray analysis since their expression was significantly lower in stool and plasma samples of patients with CRC. Moreover, receiver operating characteristic curve analysis demonstrated that fecal let-7f expression levels have significant sensitivity and specificity to distinguish between patients with CRC and healthy subjects. In conclusion, if the results are confirmed in larger series of patients, underexpressed let-7a-5p and let-7f-5p miRNAs in both plasma and stool samples of patients with CRC may serve potentially as noninvasive molecular biomarkers for the early detection of CRC.
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spelling pubmed-47113912016-01-20 Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma Ghanbari, Reza Mosakhani, Neda Sarhadi, Virinder K. Armengol, Gemma Nouraee, Nazila Mohammadkhani, Ashraf Khorrami, Samaneh Arefian, Ehsan Paryan, Mahdi Malekzadeh, Reza Knuutila, Sakari Biomark Cancer Original Research Colorectal cancer (CRC) is the third most common malignancy and the second most common cause of cancer death worldwide. Early detection of CRC can improve patient survival rates; thus, the identification of noninvasive diagnostic markers is urgently needed. MicroRNAs (miRNAs) have extensive potential to diagnose several diseases, including cancer. In this study, we compared the expression pattern of miRNAs from plasma and stool samples of patients with early stages of CRC (I, II) with that of healthy subjects. We performed miRNA profiling using microarrays on plasma and stool samples of eight patients with CRC and four healthy subjects. Seven miRNAs were found to be underexpressed in both plasma and stool samples of patients with CRC versus healthy subjects. Then, we aimed to verify two out of these seven differentially expressed miRNAs (let-7a-5p and let-7f-5p) by quantitative reverse transcriptase polymerase chain reaction on a larger set of plasma and stool samples of 51 patients with CRC and 26 healthy subjects. We confirmed the results of microarray analysis since their expression was significantly lower in stool and plasma samples of patients with CRC. Moreover, receiver operating characteristic curve analysis demonstrated that fecal let-7f expression levels have significant sensitivity and specificity to distinguish between patients with CRC and healthy subjects. In conclusion, if the results are confirmed in larger series of patients, underexpressed let-7a-5p and let-7f-5p miRNAs in both plasma and stool samples of patients with CRC may serve potentially as noninvasive molecular biomarkers for the early detection of CRC. Libertas Academica 2016-01-12 /pmc/articles/PMC4711391/ /pubmed/26793011 http://dx.doi.org/10.4137/BIC.S25252 Text en © 2016 the author(s), publisher and licensee Libertas Academica Ltd. This is an open access article published under the Creative Commons CC-BY-NC 3.0 license.
spellingShingle Original Research
Ghanbari, Reza
Mosakhani, Neda
Sarhadi, Virinder K.
Armengol, Gemma
Nouraee, Nazila
Mohammadkhani, Ashraf
Khorrami, Samaneh
Arefian, Ehsan
Paryan, Mahdi
Malekzadeh, Reza
Knuutila, Sakari
Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title_full Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title_fullStr Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title_full_unstemmed Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title_short Simultaneous Underexpression of let-7a-5p and let-7f-5p microRNAs in Plasma and Stool Samples from Early Stage Colorectal Carcinoma
title_sort simultaneous underexpression of let-7a-5p and let-7f-5p micrornas in plasma and stool samples from early stage colorectal carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711391/
https://www.ncbi.nlm.nih.gov/pubmed/26793011
http://dx.doi.org/10.4137/BIC.S25252
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