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Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse
Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Crystallographic Association
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711609/ https://www.ncbi.nlm.nih.gov/pubmed/26798803 http://dx.doi.org/10.1063/1.4919398 |
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author | Galli, L. Son, S.-K. Klinge, M. Bajt, S. Barty, A. Bean, R. Betzel, C. Beyerlein, K. R. Caleman, C. Doak, R. B. Duszenko, M. Fleckenstein, H. Gati, C. Hunt, B. Kirian, R. A. Liang, M. Nanao, M. H. Nass, K. Oberthür, D. Redecke, L. Shoeman, R. Stellato, F. Yoon, C. H. White, T. A. Yefanov, O. Spence, J. Chapman, H. N. |
author_facet | Galli, L. Son, S.-K. Klinge, M. Bajt, S. Barty, A. Bean, R. Betzel, C. Beyerlein, K. R. Caleman, C. Doak, R. B. Duszenko, M. Fleckenstein, H. Gati, C. Hunt, B. Kirian, R. A. Liang, M. Nanao, M. H. Nass, K. Oberthür, D. Redecke, L. Shoeman, R. Stellato, F. Yoon, C. H. White, T. A. Yefanov, O. Spence, J. Chapman, H. N. |
author_sort | Galli, L. |
collection | PubMed |
description | Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed. |
format | Online Article Text |
id | pubmed-4711609 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Crystallographic Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-47116092016-01-21 Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse Galli, L. Son, S.-K. Klinge, M. Bajt, S. Barty, A. Bean, R. Betzel, C. Beyerlein, K. R. Caleman, C. Doak, R. B. Duszenko, M. Fleckenstein, H. Gati, C. Hunt, B. Kirian, R. A. Liang, M. Nanao, M. H. Nass, K. Oberthür, D. Redecke, L. Shoeman, R. Stellato, F. Yoon, C. H. White, T. A. Yefanov, O. Spence, J. Chapman, H. N. Struct Dyn SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 Current hard X-ray free-electron laser (XFEL) sources can deliver doses to biological macromolecules well exceeding 1 GGy, in timescales of a few tens of femtoseconds. During the pulse, photoionization can reach the point of saturation in which certain atomic species in the sample lose most of their electrons. This electronic radiation damage causes the atomic scattering factors to change, affecting, in particular, the heavy atoms, due to their higher photoabsorption cross sections. Here, it is shown that experimental serial femtosecond crystallography data collected with an extremely bright XFEL source exhibit a reduction of the effective scattering power of the sulfur atoms in a native protein. Quantitative methods are developed to retrieve information on the effective ionization of the damaged atomic species from experimental data, and the implications of utilizing new phasing methods which can take advantage of this localized radiation damage are discussed. American Crystallographic Association 2015-04-29 /pmc/articles/PMC4711609/ /pubmed/26798803 http://dx.doi.org/10.1063/1.4919398 Text en © 2015 Author(s). 2329-7778/2015/2(4)/041703/8 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. |
spellingShingle | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 Galli, L. Son, S.-K. Klinge, M. Bajt, S. Barty, A. Bean, R. Betzel, C. Beyerlein, K. R. Caleman, C. Doak, R. B. Duszenko, M. Fleckenstein, H. Gati, C. Hunt, B. Kirian, R. A. Liang, M. Nanao, M. H. Nass, K. Oberthür, D. Redecke, L. Shoeman, R. Stellato, F. Yoon, C. H. White, T. A. Yefanov, O. Spence, J. Chapman, H. N. Electronic damage in S atoms in a native protein crystal induced by an intense X-ray free-electron laser pulse |
title | Electronic damage in S atoms in a native protein crystal induced by an
intense X-ray free-electron laser pulse |
title_full | Electronic damage in S atoms in a native protein crystal induced by an
intense X-ray free-electron laser pulse |
title_fullStr | Electronic damage in S atoms in a native protein crystal induced by an
intense X-ray free-electron laser pulse |
title_full_unstemmed | Electronic damage in S atoms in a native protein crystal induced by an
intense X-ray free-electron laser pulse |
title_short | Electronic damage in S atoms in a native protein crystal induced by an
intense X-ray free-electron laser pulse |
title_sort | electronic damage in s atoms in a native protein crystal induced by an
intense x-ray free-electron laser pulse |
topic | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711609/ https://www.ncbi.nlm.nih.gov/pubmed/26798803 http://dx.doi.org/10.1063/1.4919398 |
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