Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging

For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher i...

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Autores principales: Coughlan, H. D., Darmanin, C., Phillips, N. W., Hofmann, F., Clark, J. N., Harder, R. J., Vine, D. J., Abbey, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Crystallographic Association 2015
Materias:
SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711611/
https://www.ncbi.nlm.nih.gov/pubmed/26798804
http://dx.doi.org/10.1063/1.4919641
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author Coughlan, H. D.
Darmanin, C.
Phillips, N. W.
Hofmann, F.
Clark, J. N.
Harder, R. J.
Vine, D. J.
Abbey, B.
author_facet Coughlan, H. D.
Darmanin, C.
Phillips, N. W.
Hofmann, F.
Clark, J. N.
Harder, R. J.
Vine, D. J.
Abbey, B.
author_sort Coughlan, H. D.
collection PubMed
description For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources.
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spelling pubmed-47116112016-01-21 Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging Coughlan, H. D. Darmanin, C. Phillips, N. W. Hofmann, F. Clark, J. N. Harder, R. J. Vine, D. J. Abbey, B. Struct Dyn SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 For laboratory and synchrotron based X-ray sources, radiation damage has posed a significant barrier to obtaining high-resolution structural data from biological macromolecules. The problem is particularly acute for micron-sized crystals where the weaker signal often necessitates the use of higher intensity beams to obtain the relevant data. Here, we employ a combination of techniques, including Bragg coherent diffractive imaging to characterise the radiation induced damage in a micron-sized protein crystal over time. The approach we adopt here could help screen for potential protein crystal candidates for measurement at X-ray free election laser sources. American Crystallographic Association 2015-04-29 /pmc/articles/PMC4711611/ /pubmed/26798804 http://dx.doi.org/10.1063/1.4919641 Text en © 2015 Author(s). 2329-7778/2015/2(4)/041704/13 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
spellingShingle SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2
Coughlan, H. D.
Darmanin, C.
Phillips, N. W.
Hofmann, F.
Clark, J. N.
Harder, R. J.
Vine, D. J.
Abbey, B.
Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title_full Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title_fullStr Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title_full_unstemmed Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title_short Radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and Bragg coherent diffractive imaging
title_sort radiation damage in a micron-sized protein crystal studied via reciprocal space mapping and bragg coherent diffractive imaging
topic SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711611/
https://www.ncbi.nlm.nih.gov/pubmed/26798804
http://dx.doi.org/10.1063/1.4919641
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