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Real-time investigation of dynamic protein crystallization in living cellsa)
X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-e...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Crystallographic Association
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711630/ https://www.ncbi.nlm.nih.gov/pubmed/26798811 http://dx.doi.org/10.1063/1.4921591 |
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author | Schönherr, R. Klinge, M. Rudolph, J. M. Fita, K. Rehders, D. Lübber, F. Schneegans, S. Majoul, I. V. Duszenko, M. Betzel, C. Brandariz-Nuñez, A. Martinez-Costas, J. Duden, R. Redecke, L. |
author_facet | Schönherr, R. Klinge, M. Rudolph, J. M. Fita, K. Rehders, D. Lübber, F. Schneegans, S. Majoul, I. V. Duszenko, M. Betzel, C. Brandariz-Nuñez, A. Martinez-Costas, J. Duden, R. Redecke, L. |
author_sort | Schönherr, R. |
collection | PubMed |
description | X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro. |
format | Online Article Text |
id | pubmed-4711630 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | American Crystallographic Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-47116302016-01-21 Real-time investigation of dynamic protein crystallization in living cellsa) Schönherr, R. Klinge, M. Rudolph, J. M. Fita, K. Rehders, D. Lübber, F. Schneegans, S. Majoul, I. V. Duszenko, M. Betzel, C. Brandariz-Nuñez, A. Martinez-Costas, J. Duden, R. Redecke, L. Struct Dyn SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 X-ray crystallography requires sufficiently large crystals to obtain structural insights at atomic resolution, routinely obtained in vitro by time-consuming screening. Recently, successful data collection was reported from protein microcrystals grown within living cells using highly brilliant free-electron laser and third-generation synchrotron radiation. Here, we analyzed in vivo crystal growth of firefly luciferase and Green Fluorescent Protein-tagged reovirus μNS by live-cell imaging, showing that dimensions of living cells did not limit crystal size. The crystallization process is highly dynamic and occurs in different cellular compartments. In vivo protein crystallization offers exciting new possibilities for proteins that do not form crystals in vitro. American Crystallographic Association 2015-05-22 /pmc/articles/PMC4711630/ /pubmed/26798811 http://dx.doi.org/10.1063/1.4921591 Text en © 2015 Author(s). 2329-7778/2015/2(4)/041712/17 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. |
spellingShingle | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 Schönherr, R. Klinge, M. Rudolph, J. M. Fita, K. Rehders, D. Lübber, F. Schneegans, S. Majoul, I. V. Duszenko, M. Betzel, C. Brandariz-Nuñez, A. Martinez-Costas, J. Duden, R. Redecke, L. Real-time investigation of dynamic protein crystallization in living cellsa) |
title | Real-time investigation of dynamic protein crystallization in living cellsa) |
title_full | Real-time investigation of dynamic protein crystallization in living cellsa) |
title_fullStr | Real-time investigation of dynamic protein crystallization in living cellsa) |
title_full_unstemmed | Real-time investigation of dynamic protein crystallization in living cellsa) |
title_short | Real-time investigation of dynamic protein crystallization in living cellsa) |
title_sort | real-time investigation of dynamic protein crystallization in living cellsa) |
topic | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711630/ https://www.ncbi.nlm.nih.gov/pubmed/26798811 http://dx.doi.org/10.1063/1.4921591 |
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