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Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers
A major challenge in high-resolution x-ray free-electron laser-based coherent diffractive imaging is the development of aerosol injectors that can efficiently deliver particles to the peak intensity of the focused X-ray beam. Here, we consider the use of a simple convergent-orifice nozzle for produc...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Crystallographic Association
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711644/ https://www.ncbi.nlm.nih.gov/pubmed/26798816 http://dx.doi.org/10.1063/1.4922648 |
| _version_ | 1782409965677314048 |
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| author | Kirian, R. A. Awel, S. Eckerskorn, N. Fleckenstein, H. Wiedorn, M. Adriano, L. Bajt, S. Barthelmess, M. Bean, R. Beyerlein, K. R. Chavas, L. M. G. Domaracky, M. Heymann, M. Horke, D. A. Knoska, J. Metz, M. Morgan, A. Oberthuer, D. Roth, N. Sato, T. Xavier, P. L. Yefanov, O. Rode, A. V. Küpper, J. Chapman, H. N. |
| author_facet | Kirian, R. A. Awel, S. Eckerskorn, N. Fleckenstein, H. Wiedorn, M. Adriano, L. Bajt, S. Barthelmess, M. Bean, R. Beyerlein, K. R. Chavas, L. M. G. Domaracky, M. Heymann, M. Horke, D. A. Knoska, J. Metz, M. Morgan, A. Oberthuer, D. Roth, N. Sato, T. Xavier, P. L. Yefanov, O. Rode, A. V. Küpper, J. Chapman, H. N. |
| author_sort | Kirian, R. A. |
| collection | PubMed |
| description | A major challenge in high-resolution x-ray free-electron laser-based coherent diffractive imaging is the development of aerosol injectors that can efficiently deliver particles to the peak intensity of the focused X-ray beam. Here, we consider the use of a simple convergent-orifice nozzle for producing tightly focused beams of particles. Through optical imaging we show that 0.5 μm particles can be focused to a full-width at half maximum diameter of 4.2 μm, and we demonstrate the use of such a nozzle for injecting viruses into a micro-focused soft-X-ray FEL beam. |
| format | Online Article Text |
| id | pubmed-4711644 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | American Crystallographic Association |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-47116442016-01-21 Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers Kirian, R. A. Awel, S. Eckerskorn, N. Fleckenstein, H. Wiedorn, M. Adriano, L. Bajt, S. Barthelmess, M. Bean, R. Beyerlein, K. R. Chavas, L. M. G. Domaracky, M. Heymann, M. Horke, D. A. Knoska, J. Metz, M. Morgan, A. Oberthuer, D. Roth, N. Sato, T. Xavier, P. L. Yefanov, O. Rode, A. V. Küpper, J. Chapman, H. N. Struct Dyn SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 A major challenge in high-resolution x-ray free-electron laser-based coherent diffractive imaging is the development of aerosol injectors that can efficiently deliver particles to the peak intensity of the focused X-ray beam. Here, we consider the use of a simple convergent-orifice nozzle for producing tightly focused beams of particles. Through optical imaging we show that 0.5 μm particles can be focused to a full-width at half maximum diameter of 4.2 μm, and we demonstrate the use of such a nozzle for injecting viruses into a micro-focused soft-X-ray FEL beam. American Crystallographic Association 2015-06-19 /pmc/articles/PMC4711644/ /pubmed/26798816 http://dx.doi.org/10.1063/1.4922648 Text en © 2015 Author(s). 2329-7778/2015/2(4)/041717/12 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License. |
| spellingShingle | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 Kirian, R. A. Awel, S. Eckerskorn, N. Fleckenstein, H. Wiedorn, M. Adriano, L. Bajt, S. Barthelmess, M. Bean, R. Beyerlein, K. R. Chavas, L. M. G. Domaracky, M. Heymann, M. Horke, D. A. Knoska, J. Metz, M. Morgan, A. Oberthuer, D. Roth, N. Sato, T. Xavier, P. L. Yefanov, O. Rode, A. V. Küpper, J. Chapman, H. N. Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title | Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title_full | Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title_fullStr | Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title_full_unstemmed | Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title_short | Simple convergent-nozzle aerosol injector for single-particle diffractive imaging with X-ray free-electron lasers |
| title_sort | simple convergent-nozzle aerosol injector for single-particle diffractive imaging with x-ray free-electron lasers |
| topic | SPECIAL TOPIC: BIOLOGY WITH X-RAY LASERS 2 |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4711644/ https://www.ncbi.nlm.nih.gov/pubmed/26798816 http://dx.doi.org/10.1063/1.4922648 |
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