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Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century

Ancient DNA (aDNA) recovered from plague victims of the second plague pandemic (14(th) to 17(th) century), excavated from two different burial sites in Germany, and spanning a time period of more than 300 years, was characterized using single nucleotide polymorphism (SNP) analysis. Of 30 tested skel...

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Autores principales: Seifert, Lisa, Wiechmann, Ingrid, Harbeck, Michaela, Thomas, Astrid, Grupe, Gisela, Projahn, Michaela, Scholz, Holger C., Riehm, Julia M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712009/
https://www.ncbi.nlm.nih.gov/pubmed/26760973
http://dx.doi.org/10.1371/journal.pone.0145194
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author Seifert, Lisa
Wiechmann, Ingrid
Harbeck, Michaela
Thomas, Astrid
Grupe, Gisela
Projahn, Michaela
Scholz, Holger C.
Riehm, Julia M.
author_facet Seifert, Lisa
Wiechmann, Ingrid
Harbeck, Michaela
Thomas, Astrid
Grupe, Gisela
Projahn, Michaela
Scholz, Holger C.
Riehm, Julia M.
author_sort Seifert, Lisa
collection PubMed
description Ancient DNA (aDNA) recovered from plague victims of the second plague pandemic (14(th) to 17(th) century), excavated from two different burial sites in Germany, and spanning a time period of more than 300 years, was characterized using single nucleotide polymorphism (SNP) analysis. Of 30 tested skeletons 8 were positive for Yersinia pestis-specific nucleic acid, as determined by qPCR targeting the pla gene. In one individual (MP-19-II), the pla copy number in DNA extracted from tooth pulp was as high as 700 gene copies/μl, indicating severe generalized infection. All positive individuals were identical in all 16 SNP positions, separating phylogenetic branches within nodes N07_N10 (14 SNPs), N07_N08 (SNP s19) and N06_N07 (s545), and were highly similar to previously investigated plague victims from other European countries. Thus, beside the assumed continuous reintroduction of Y. pestis from central Asia in multiple waves during the second pandemic, long-term persistence of Y. pestis in Europe in a yet unknown reservoir host has also to be considered.
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spelling pubmed-47120092016-01-26 Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century Seifert, Lisa Wiechmann, Ingrid Harbeck, Michaela Thomas, Astrid Grupe, Gisela Projahn, Michaela Scholz, Holger C. Riehm, Julia M. PLoS One Research Article Ancient DNA (aDNA) recovered from plague victims of the second plague pandemic (14(th) to 17(th) century), excavated from two different burial sites in Germany, and spanning a time period of more than 300 years, was characterized using single nucleotide polymorphism (SNP) analysis. Of 30 tested skeletons 8 were positive for Yersinia pestis-specific nucleic acid, as determined by qPCR targeting the pla gene. In one individual (MP-19-II), the pla copy number in DNA extracted from tooth pulp was as high as 700 gene copies/μl, indicating severe generalized infection. All positive individuals were identical in all 16 SNP positions, separating phylogenetic branches within nodes N07_N10 (14 SNPs), N07_N08 (SNP s19) and N06_N07 (s545), and were highly similar to previously investigated plague victims from other European countries. Thus, beside the assumed continuous reintroduction of Y. pestis from central Asia in multiple waves during the second pandemic, long-term persistence of Y. pestis in Europe in a yet unknown reservoir host has also to be considered. Public Library of Science 2016-01-13 /pmc/articles/PMC4712009/ /pubmed/26760973 http://dx.doi.org/10.1371/journal.pone.0145194 Text en © 2016 Seifert et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Article
Seifert, Lisa
Wiechmann, Ingrid
Harbeck, Michaela
Thomas, Astrid
Grupe, Gisela
Projahn, Michaela
Scholz, Holger C.
Riehm, Julia M.
Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title_full Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title_fullStr Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title_full_unstemmed Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title_short Genotyping Yersinia pestis in Historical Plague: Evidence for Long-Term Persistence of Y. pestis in Europe from the 14(th) to the 17(th) Century
title_sort genotyping yersinia pestis in historical plague: evidence for long-term persistence of y. pestis in europe from the 14(th) to the 17(th) century
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712009/
https://www.ncbi.nlm.nih.gov/pubmed/26760973
http://dx.doi.org/10.1371/journal.pone.0145194
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