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Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology

The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16). The assessment of stability and functionality of s...

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Autores principales: Padilla-Morales, Luis F., Colón-Sáez, José O., González-Nieves, Joel E., Quesada-González, Orestes, Lasalde-Dominicci, José A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712261/
https://www.ncbi.nlm.nih.gov/pubmed/26870753
http://dx.doi.org/10.1016/j.dib.2015.12.010
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author Padilla-Morales, Luis F.
Colón-Sáez, José O.
González-Nieves, Joel E.
Quesada-González, Orestes
Lasalde-Dominicci, José A.
author_facet Padilla-Morales, Luis F.
Colón-Sáez, José O.
González-Nieves, Joel E.
Quesada-González, Orestes
Lasalde-Dominicci, José A.
author_sort Padilla-Morales, Luis F.
collection PubMed
description The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16). The assessment of stability and functionality of solubilized membrane protein is a critical step prior to further crystallization trails. One of the key factors for this task is the appropriate choice of a detergent that can support nAChR activity and stability comparable to the crude membranes. The stability of the nAChR-LFG-16 complex incorporated into lipid cubic phase (LCP) was monitored for a period of 30 days by means of fluorescence recovery after photobleaching (FRAP) and the functionality was evaluated after its incorporation into Xenopus oocyte by means of the two electrode voltage clamp technique.
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spelling pubmed-47122612016-02-11 Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology Padilla-Morales, Luis F. Colón-Sáez, José O. González-Nieves, Joel E. Quesada-González, Orestes Lasalde-Dominicci, José A. Data Brief Data Article The presented data provides additional information about the assessment of affinity purified nicotinic acetylcholine receptor (nAChR) rich membrane solubilized with long chain (16 saturated carbons) lysophospholipid with glycerol headgroup (LFG-16). The assessment of stability and functionality of solubilized membrane protein is a critical step prior to further crystallization trails. One of the key factors for this task is the appropriate choice of a detergent that can support nAChR activity and stability comparable to the crude membranes. The stability of the nAChR-LFG-16 complex incorporated into lipid cubic phase (LCP) was monitored for a period of 30 days by means of fluorescence recovery after photobleaching (FRAP) and the functionality was evaluated after its incorporation into Xenopus oocyte by means of the two electrode voltage clamp technique. Elsevier 2015-12-25 /pmc/articles/PMC4712261/ /pubmed/26870753 http://dx.doi.org/10.1016/j.dib.2015.12.010 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Padilla-Morales, Luis F.
Colón-Sáez, José O.
González-Nieves, Joel E.
Quesada-González, Orestes
Lasalde-Dominicci, José A.
Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title_full Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title_fullStr Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title_full_unstemmed Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title_short Functionality and stability data of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology
title_sort functionality and stability data of detergent purified nachr from torpedo using lipidic matrixes and macroscopic electrophysiology
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712261/
https://www.ncbi.nlm.nih.gov/pubmed/26870753
http://dx.doi.org/10.1016/j.dib.2015.12.010
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