Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli

BACKGROUND: Significant challenges, including low expression and extracellular secretion of soluble protein, are encountered in expressing and purifying Bacillus acidopullulyticus pullulanase (BaPul) in Escherichia coli. METHODS: An N-terminal domain truncation was adopted to facilitate BaPul varian...

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Detalles Bibliográficos
Autores principales: Chen, Ana, Sun, Yang, Zhang, Wei, Peng, Feng, Zhan, Chunjun, Liu, Meng, Yang, Yankun, Bai, Zhonghu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712565/
https://www.ncbi.nlm.nih.gov/pubmed/26762529
http://dx.doi.org/10.1186/s12934-015-0403-5

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