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Altered miRNA expression in pulmonary sarcoidosis
BACKGROUND: miRNAs control important cellular functions including angiogenesis/angiostasis or fibrosis and reveal altered expression during pathological processes in the lung. METHODS: The aim of the study was to investigate the expression of selected miRNAs (miR-let7f, miR-15b, miR-16, miR-20a, miR...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712597/ https://www.ncbi.nlm.nih.gov/pubmed/26768132 http://dx.doi.org/10.1186/s12881-016-0266-6 |
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author | Kiszałkiewicz, Justyna Piotrowski, Wojciech J. Pastuszak-Lewandoska, Dorota Górski, Paweł Antczak, Adam Górski, Witold Domańska-Senderowska, Daria Migdalska-Sęk, Monika Czarnecka, Karolina H. Nawrot, Ewa Brzeziańska-Lasota, Ewa |
author_facet | Kiszałkiewicz, Justyna Piotrowski, Wojciech J. Pastuszak-Lewandoska, Dorota Górski, Paweł Antczak, Adam Górski, Witold Domańska-Senderowska, Daria Migdalska-Sęk, Monika Czarnecka, Karolina H. Nawrot, Ewa Brzeziańska-Lasota, Ewa |
author_sort | Kiszałkiewicz, Justyna |
collection | PubMed |
description | BACKGROUND: miRNAs control important cellular functions including angiogenesis/angiostasis or fibrosis and reveal altered expression during pathological processes in the lung. METHODS: The aim of the study was to investigate the expression of selected miRNAs (miR-let7f, miR-15b, miR-16, miR-20a, miR-27b, miR-128a, miR-130a, miR-192 miR-221, miR-222) in patients with pulmonary sarcoidosis (n = 94) and controls (n = 50). The expression was assessed by q-PCR in BALF cells and peripheral blood lymphocytes (PB lymphocytes). For statistical analysis, the Kruskal–Wallis test, Mann–Whitney U- test, Neuman–Keuls’ multiple comparison test, and Spearman’s rank correlation were used. RESULTS: In BALF cells, significantly higher expression of miR-192 and miR-221 and lower expression of miR-15b were found in patients than controls. MiR-27b, miR-192 and miR-221 expression was significantly higher in patients without parenchymal involvement (stages I) than those at stages II-IV. Patients with acute disease demonstrated significantly higher miR-27b, miR-192 and miR-221 expression than those with insidious onset. For PB lymphocytes, patients demonstrated significantly greater miR-15b, miR-27b, miR-192, miR-221 and miR-222 expression, but lower miR-let7f and miR-130a expression, than controls. Stage I patients demonstrated significantly higher miR-16 and miR-15b expression than those in stages II-IV, and patients with the acute form demonstrated higher miR-130a and miR-15b expression. In BALF cells, miR-16 and miR-20a expression was significantly higher in patients with lung volume restriction, and miR-let7f was higher in the PB lymphocytes in patients with obturation. Several correlations were observed between the pattern of miRNA expression, lung function parameters and selected laboratory markers. CONCLUSION: The obtained results suggest that the studied miRNAs play a role in the pathogenesis of sarcoidosis, and that some of them might have negative prognostic value. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12881-016-0266-6) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4712597 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47125972016-01-15 Altered miRNA expression in pulmonary sarcoidosis Kiszałkiewicz, Justyna Piotrowski, Wojciech J. Pastuszak-Lewandoska, Dorota Górski, Paweł Antczak, Adam Górski, Witold Domańska-Senderowska, Daria Migdalska-Sęk, Monika Czarnecka, Karolina H. Nawrot, Ewa Brzeziańska-Lasota, Ewa BMC Med Genet Research Article BACKGROUND: miRNAs control important cellular functions including angiogenesis/angiostasis or fibrosis and reveal altered expression during pathological processes in the lung. METHODS: The aim of the study was to investigate the expression of selected miRNAs (miR-let7f, miR-15b, miR-16, miR-20a, miR-27b, miR-128a, miR-130a, miR-192 miR-221, miR-222) in patients with pulmonary sarcoidosis (n = 94) and controls (n = 50). The expression was assessed by q-PCR in BALF cells and peripheral blood lymphocytes (PB lymphocytes). For statistical analysis, the Kruskal–Wallis test, Mann–Whitney U- test, Neuman–Keuls’ multiple comparison test, and Spearman’s rank correlation were used. RESULTS: In BALF cells, significantly higher expression of miR-192 and miR-221 and lower expression of miR-15b were found in patients than controls. MiR-27b, miR-192 and miR-221 expression was significantly higher in patients without parenchymal involvement (stages I) than those at stages II-IV. Patients with acute disease demonstrated significantly higher miR-27b, miR-192 and miR-221 expression than those with insidious onset. For PB lymphocytes, patients demonstrated significantly greater miR-15b, miR-27b, miR-192, miR-221 and miR-222 expression, but lower miR-let7f and miR-130a expression, than controls. Stage I patients demonstrated significantly higher miR-16 and miR-15b expression than those in stages II-IV, and patients with the acute form demonstrated higher miR-130a and miR-15b expression. In BALF cells, miR-16 and miR-20a expression was significantly higher in patients with lung volume restriction, and miR-let7f was higher in the PB lymphocytes in patients with obturation. Several correlations were observed between the pattern of miRNA expression, lung function parameters and selected laboratory markers. CONCLUSION: The obtained results suggest that the studied miRNAs play a role in the pathogenesis of sarcoidosis, and that some of them might have negative prognostic value. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12881-016-0266-6) contains supplementary material, which is available to authorized users. BioMed Central 2016-01-14 /pmc/articles/PMC4712597/ /pubmed/26768132 http://dx.doi.org/10.1186/s12881-016-0266-6 Text en © Kiszałkiewicz et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Kiszałkiewicz, Justyna Piotrowski, Wojciech J. Pastuszak-Lewandoska, Dorota Górski, Paweł Antczak, Adam Górski, Witold Domańska-Senderowska, Daria Migdalska-Sęk, Monika Czarnecka, Karolina H. Nawrot, Ewa Brzeziańska-Lasota, Ewa Altered miRNA expression in pulmonary sarcoidosis |
title | Altered miRNA expression in pulmonary sarcoidosis |
title_full | Altered miRNA expression in pulmonary sarcoidosis |
title_fullStr | Altered miRNA expression in pulmonary sarcoidosis |
title_full_unstemmed | Altered miRNA expression in pulmonary sarcoidosis |
title_short | Altered miRNA expression in pulmonary sarcoidosis |
title_sort | altered mirna expression in pulmonary sarcoidosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712597/ https://www.ncbi.nlm.nih.gov/pubmed/26768132 http://dx.doi.org/10.1186/s12881-016-0266-6 |
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