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Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts

The Follicle-Stimulating Hormone Receptor (FSHR) is used as an imaging biomarker for the detection of ovarian cancer (OC). FSHR is highly expressed on ovarian tumors and involved with cancer development and metastatic signaling pathways. A decapeptide specific to the FSHR extracellular domain is syn...

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Autores principales: Lee, Chung-Wein, Guo, Lili, Matei, Daniela, Stantz, Keith
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712933/
https://www.ncbi.nlm.nih.gov/pubmed/26779384
http://dx.doi.org/10.4172/2155-952X.1000198
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author Lee, Chung-Wein
Guo, Lili
Matei, Daniela
Stantz, Keith
author_facet Lee, Chung-Wein
Guo, Lili
Matei, Daniela
Stantz, Keith
author_sort Lee, Chung-Wein
collection PubMed
description The Follicle-Stimulating Hormone Receptor (FSHR) is used as an imaging biomarker for the detection of ovarian cancer (OC). FSHR is highly expressed on ovarian tumors and involved with cancer development and metastatic signaling pathways. A decapeptide specific to the FSHR extracellular domain is synthesized and conjugated to fluorescent dyes to image OC cells in vitro and tumors xenograft model in vivo. The in vitro binding curve and the average number of FSHR per cell are obtained for OVCAR-3 cells by a high resolution flow cytometer. For the decapeptide, the measured EC50 was 160 μM and the average number of receptors per cell was 1.7 × 10(7). The decapeptide molecular imaging probe reached a maximum tumor to muscle ratio five hours after intravenous injection and a dose-dependent plateau after 24–48 hours. These results indicate the potential application of a small molecular weight imaging probe specific to ovarian cancer through binding to FSHR. Based on these results, multimeric constructs are being developed to optimize binding to ovarian cells and tumors.
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spelling pubmed-47129332016-01-14 Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts Lee, Chung-Wein Guo, Lili Matei, Daniela Stantz, Keith J Biotechnol Biomater Article The Follicle-Stimulating Hormone Receptor (FSHR) is used as an imaging biomarker for the detection of ovarian cancer (OC). FSHR is highly expressed on ovarian tumors and involved with cancer development and metastatic signaling pathways. A decapeptide specific to the FSHR extracellular domain is synthesized and conjugated to fluorescent dyes to image OC cells in vitro and tumors xenograft model in vivo. The in vitro binding curve and the average number of FSHR per cell are obtained for OVCAR-3 cells by a high resolution flow cytometer. For the decapeptide, the measured EC50 was 160 μM and the average number of receptors per cell was 1.7 × 10(7). The decapeptide molecular imaging probe reached a maximum tumor to muscle ratio five hours after intravenous injection and a dose-dependent plateau after 24–48 hours. These results indicate the potential application of a small molecular weight imaging probe specific to ovarian cancer through binding to FSHR. Based on these results, multimeric constructs are being developed to optimize binding to ovarian cells and tumors. 2015-09-10 2015-09 /pmc/articles/PMC4712933/ /pubmed/26779384 http://dx.doi.org/10.4172/2155-952X.1000198 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Lee, Chung-Wein
Guo, Lili
Matei, Daniela
Stantz, Keith
Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title_full Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title_fullStr Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title_full_unstemmed Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title_short Development of Follicle-Stimulating Hormone Receptor Binding Probes to Image Ovarian Xenografts
title_sort development of follicle-stimulating hormone receptor binding probes to image ovarian xenografts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4712933/
https://www.ncbi.nlm.nih.gov/pubmed/26779384
http://dx.doi.org/10.4172/2155-952X.1000198
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