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Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1

The Golgi apparatus is the central hub of intracellular trafficking and consists of tethered stacks of cis, medial, and trans cisternae. In mammalian cells, these cisternae are stitched together as a perinuclear Golgi ribbon, which is required for the establishment of cell polarity and normal subcel...

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Detalles Bibliográficos
Autores principales: Copeland, Sarah J., Thurston, Susan F., Copeland, John W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713130/
https://www.ncbi.nlm.nih.gov/pubmed/26564798
http://dx.doi.org/10.1091/mbc.E15-02-0070
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author Copeland, Sarah J.
Thurston, Susan F.
Copeland, John W.
author_facet Copeland, Sarah J.
Thurston, Susan F.
Copeland, John W.
author_sort Copeland, Sarah J.
collection PubMed
description The Golgi apparatus is the central hub of intracellular trafficking and consists of tethered stacks of cis, medial, and trans cisternae. In mammalian cells, these cisternae are stitched together as a perinuclear Golgi ribbon, which is required for the establishment of cell polarity and normal subcellular organization. We previously identified FHDC1 (also known as INF1) as a unique microtubule-binding member of the formin family of cytoskeletal-remodeling proteins. We show here that endogenous FHDC1 regulates Golgi ribbon formation and has an apparent preferential association with the Golgi-derived microtubule network. Knockdown of FHDC1 expression results in defective Golgi assembly and suggests a role for FHDC1 in maintenance of the Golgi-derived microtubule network. Similarly, overexpression of FHDC1 induces dispersion of the Golgi ribbon into functional ministacks. This effect is independent of centrosome-derived microtubules and instead likely requires the interaction between the FHDC1 microtubule-binding domain and the Golgi-derived microtubule network. These effects also depend on the interaction between the FHDC1 FH2 domain and the actin cytoskeleton. Thus our results suggest that the coordination of actin and microtubule dynamics by FHDC1 is required for normal Golgi ribbon formation.
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spelling pubmed-47131302016-03-30 Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1 Copeland, Sarah J. Thurston, Susan F. Copeland, John W. Mol Biol Cell Articles The Golgi apparatus is the central hub of intracellular trafficking and consists of tethered stacks of cis, medial, and trans cisternae. In mammalian cells, these cisternae are stitched together as a perinuclear Golgi ribbon, which is required for the establishment of cell polarity and normal subcellular organization. We previously identified FHDC1 (also known as INF1) as a unique microtubule-binding member of the formin family of cytoskeletal-remodeling proteins. We show here that endogenous FHDC1 regulates Golgi ribbon formation and has an apparent preferential association with the Golgi-derived microtubule network. Knockdown of FHDC1 expression results in defective Golgi assembly and suggests a role for FHDC1 in maintenance of the Golgi-derived microtubule network. Similarly, overexpression of FHDC1 induces dispersion of the Golgi ribbon into functional ministacks. This effect is independent of centrosome-derived microtubules and instead likely requires the interaction between the FHDC1 microtubule-binding domain and the Golgi-derived microtubule network. These effects also depend on the interaction between the FHDC1 FH2 domain and the actin cytoskeleton. Thus our results suggest that the coordination of actin and microtubule dynamics by FHDC1 is required for normal Golgi ribbon formation. The American Society for Cell Biology 2016-01-15 /pmc/articles/PMC4713130/ /pubmed/26564798 http://dx.doi.org/10.1091/mbc.E15-02-0070 Text en © 2016 Copeland et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Copeland, Sarah J.
Thurston, Susan F.
Copeland, John W.
Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title_full Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title_fullStr Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title_full_unstemmed Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title_short Actin- and microtubule-dependent regulation of Golgi morphology by FHDC1
title_sort actin- and microtubule-dependent regulation of golgi morphology by fhdc1
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713130/
https://www.ncbi.nlm.nih.gov/pubmed/26564798
http://dx.doi.org/10.1091/mbc.E15-02-0070
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