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Disruption of PHO13 improves ethanol production via the xylose isomerase pathway
Xylose is the second most abundant sugar in lignocellulosic materials and can be converted to ethanol by recombinant Saccharomyces cerevisiae yeast strains expressing heterologous genes involved in xylose assimilation pathways. Recent research demonstrated that disruption of the alkaline phosphatase...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713403/ https://www.ncbi.nlm.nih.gov/pubmed/26769491 http://dx.doi.org/10.1186/s13568-015-0175-7 |
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author | Bamba, Takahiro Hasunuma, Tomohisa Kondo, Akihiko |
author_facet | Bamba, Takahiro Hasunuma, Tomohisa Kondo, Akihiko |
author_sort | Bamba, Takahiro |
collection | PubMed |
description | Xylose is the second most abundant sugar in lignocellulosic materials and can be converted to ethanol by recombinant Saccharomyces cerevisiae yeast strains expressing heterologous genes involved in xylose assimilation pathways. Recent research demonstrated that disruption of the alkaline phosphatase gene, PHO13, enhances ethanol production from xylose by a strain expressing the xylose reductase (XR) and xylitol dehydrogenase (XDH) genes; however, the yield of ethanol is poor. In this study, PHO13 was disrupted in a recombinant strain harboring multiple copies of the xylose isomerase (XI) gene derived from Orpinomyces sp., coupled with overexpression of the endogenous xylulokinase (XK) gene and disruption of GRE3, which encodes aldose reductase. The resulting YΔGP/XK/XI strain consumed 2.08 g/L/h of xylose and produced 0.88 g/L/h of volumetric ethanol, for an 86.8 % theoretical ethanol yield, and only YΔGP/XK/XI demonstrated increase in cell concentration. Transcriptome analysis indicated that expression of genes involved in the pentose phosphate pathway (GND1, SOL3, TAL1, RKI1, and TKL1) and TCA cycle and respiratory chain (NDE1, ACO1, ACO2, SDH2, IDH1, IDH2, ATP7, ATP19, SDH4, SDH3, CMC2, and ATP15) was upregulated in the YΔGP/XK/XI strain. And the expression levels of 125 cell cycle genes were changed by deletion of PHO13. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-015-0175-7) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4713403 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47134032016-01-31 Disruption of PHO13 improves ethanol production via the xylose isomerase pathway Bamba, Takahiro Hasunuma, Tomohisa Kondo, Akihiko AMB Express Original Article Xylose is the second most abundant sugar in lignocellulosic materials and can be converted to ethanol by recombinant Saccharomyces cerevisiae yeast strains expressing heterologous genes involved in xylose assimilation pathways. Recent research demonstrated that disruption of the alkaline phosphatase gene, PHO13, enhances ethanol production from xylose by a strain expressing the xylose reductase (XR) and xylitol dehydrogenase (XDH) genes; however, the yield of ethanol is poor. In this study, PHO13 was disrupted in a recombinant strain harboring multiple copies of the xylose isomerase (XI) gene derived from Orpinomyces sp., coupled with overexpression of the endogenous xylulokinase (XK) gene and disruption of GRE3, which encodes aldose reductase. The resulting YΔGP/XK/XI strain consumed 2.08 g/L/h of xylose and produced 0.88 g/L/h of volumetric ethanol, for an 86.8 % theoretical ethanol yield, and only YΔGP/XK/XI demonstrated increase in cell concentration. Transcriptome analysis indicated that expression of genes involved in the pentose phosphate pathway (GND1, SOL3, TAL1, RKI1, and TKL1) and TCA cycle and respiratory chain (NDE1, ACO1, ACO2, SDH2, IDH1, IDH2, ATP7, ATP19, SDH4, SDH3, CMC2, and ATP15) was upregulated in the YΔGP/XK/XI strain. And the expression levels of 125 cell cycle genes were changed by deletion of PHO13. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13568-015-0175-7) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2016-01-14 /pmc/articles/PMC4713403/ /pubmed/26769491 http://dx.doi.org/10.1186/s13568-015-0175-7 Text en © Bamba et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article Bamba, Takahiro Hasunuma, Tomohisa Kondo, Akihiko Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title | Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title_full | Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title_fullStr | Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title_full_unstemmed | Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title_short | Disruption of PHO13 improves ethanol production via the xylose isomerase pathway |
title_sort | disruption of pho13 improves ethanol production via the xylose isomerase pathway |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4713403/ https://www.ncbi.nlm.nih.gov/pubmed/26769491 http://dx.doi.org/10.1186/s13568-015-0175-7 |
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