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Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor
Metastatic breast cancer remains a highly lethal disease, and it is very important to evaluate the biomarkers associated with the distant metastasis. MicroRNA (miRNA) are small non‐protein coding RNA that regulate various cellular functions. Recent investigations have demonstrated the importance of...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714682/ https://www.ncbi.nlm.nih.gov/pubmed/26331797 http://dx.doi.org/10.1111/cas.12808 |
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author | Minemura, Hiroyuki Takagi, Kiyoshi Miki, Yasuhiro Shibahara, Yukiko Nakagawa, Saki Ebata, Akiko Watanabe, Mika Ishida, Takanori Sasano, Hironobu Suzuki, Takashi |
author_facet | Minemura, Hiroyuki Takagi, Kiyoshi Miki, Yasuhiro Shibahara, Yukiko Nakagawa, Saki Ebata, Akiko Watanabe, Mika Ishida, Takanori Sasano, Hironobu Suzuki, Takashi |
author_sort | Minemura, Hiroyuki |
collection | PubMed |
description | Metastatic breast cancer remains a highly lethal disease, and it is very important to evaluate the biomarkers associated with the distant metastasis. MicroRNA (miRNA) are small non‐protein coding RNA that regulate various cellular functions. Recent investigations have demonstrated the importance of some miRNA in breast cancer, but the significance of the great majority of miRNA remains largely unclear in breast cancer metastasis. Therefore, in this study, we first examined expression profiles of miRNA in stage IV breast carcinoma tissues, comparing stage I–III cases by miRNA PCR array, and identified miR‐1 as the miRNA which was the most associated with the distant metastasis. However, miR‐1 has not yet been examined in breast carcinoma tissue, and its significance remains unknown. Therefore, we further examined miR‐1 expression in breast carcinoma using in situ hybridization (ISH). miR‐1 was localized in carcinoma cells in 20% of breast carcinoma cases, but it was negligible in non‐neoplastic mammary glands or stroma. miR‐1 ISH status was significantly associated with stage, pathological T factor, lymph node metastasis, distant metastasis, histological grade, estrogen receptor, progesterone receptor and Ki‐67 in breast carcinoma. Moreover, the miR‐1 status was demonstrated using multivariate analysis as an independent worse prognostic factor for both disease‐free and breast cancer‐specific survival. These findings suggest that abnormal miR‐1 expression is associated with an aggressive phenotype of breast carcinoma and that miR‐1 status is a potent prognostic factor in human breast cancer patients. |
format | Online Article Text |
id | pubmed-4714682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47146822016-01-22 Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor Minemura, Hiroyuki Takagi, Kiyoshi Miki, Yasuhiro Shibahara, Yukiko Nakagawa, Saki Ebata, Akiko Watanabe, Mika Ishida, Takanori Sasano, Hironobu Suzuki, Takashi Cancer Sci Original Articles Metastatic breast cancer remains a highly lethal disease, and it is very important to evaluate the biomarkers associated with the distant metastasis. MicroRNA (miRNA) are small non‐protein coding RNA that regulate various cellular functions. Recent investigations have demonstrated the importance of some miRNA in breast cancer, but the significance of the great majority of miRNA remains largely unclear in breast cancer metastasis. Therefore, in this study, we first examined expression profiles of miRNA in stage IV breast carcinoma tissues, comparing stage I–III cases by miRNA PCR array, and identified miR‐1 as the miRNA which was the most associated with the distant metastasis. However, miR‐1 has not yet been examined in breast carcinoma tissue, and its significance remains unknown. Therefore, we further examined miR‐1 expression in breast carcinoma using in situ hybridization (ISH). miR‐1 was localized in carcinoma cells in 20% of breast carcinoma cases, but it was negligible in non‐neoplastic mammary glands or stroma. miR‐1 ISH status was significantly associated with stage, pathological T factor, lymph node metastasis, distant metastasis, histological grade, estrogen receptor, progesterone receptor and Ki‐67 in breast carcinoma. Moreover, the miR‐1 status was demonstrated using multivariate analysis as an independent worse prognostic factor for both disease‐free and breast cancer‐specific survival. These findings suggest that abnormal miR‐1 expression is associated with an aggressive phenotype of breast carcinoma and that miR‐1 status is a potent prognostic factor in human breast cancer patients. John Wiley and Sons Inc. 2015-10-07 2015-11 /pmc/articles/PMC4714682/ /pubmed/26331797 http://dx.doi.org/10.1111/cas.12808 Text en © 2015 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Minemura, Hiroyuki Takagi, Kiyoshi Miki, Yasuhiro Shibahara, Yukiko Nakagawa, Saki Ebata, Akiko Watanabe, Mika Ishida, Takanori Sasano, Hironobu Suzuki, Takashi Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title | Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title_full | Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title_fullStr | Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title_full_unstemmed | Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title_short | Abnormal expression of miR‐1 in breast carcinoma as a potent prognostic factor |
title_sort | abnormal expression of mir‐1 in breast carcinoma as a potent prognostic factor |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714682/ https://www.ncbi.nlm.nih.gov/pubmed/26331797 http://dx.doi.org/10.1111/cas.12808 |
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