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Ion channels and calcium signaling in motile cilia

The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration...

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Detalles Bibliográficos
Autores principales: Doerner, Julia F, Delling, Markus, Clapham, David E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714969/
https://www.ncbi.nlm.nih.gov/pubmed/26650848
http://dx.doi.org/10.7554/eLife.11066
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author Doerner, Julia F
Delling, Markus
Clapham, David E
author_facet Doerner, Julia F
Delling, Markus
Clapham, David E
author_sort Doerner, Julia F
collection PubMed
description The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration ([Ca(2+)] ~170 nM) is only slightly elevated over cytoplasmic [Ca(2+)] (~100 nM) at steady state. Ca(2+) changes that arise in the cytoplasm rapidly equilibrate in motile cilia. We measured Ca(V)1 voltage-gated calcium channels in ependymal cells, but these channels are not specifically enriched in motile cilia. Membrane depolarization increases ciliary [Ca(2+)], but only marginally alters cilia beating and cilia-driven fluid velocity within short (~1 min) time frames. We conclude that beating of ependymal motile cilia is not tightly regulated by voltage-gated calcium channels, unlike that of well-studied motile cilia and flagella in protists, such as Paramecia and Chlamydomonas. DOI: http://dx.doi.org/10.7554/eLife.11066.001
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spelling pubmed-47149692016-01-19 Ion channels and calcium signaling in motile cilia Doerner, Julia F Delling, Markus Clapham, David E eLife Biophysics and Structural Biology The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration ([Ca(2+)] ~170 nM) is only slightly elevated over cytoplasmic [Ca(2+)] (~100 nM) at steady state. Ca(2+) changes that arise in the cytoplasm rapidly equilibrate in motile cilia. We measured Ca(V)1 voltage-gated calcium channels in ependymal cells, but these channels are not specifically enriched in motile cilia. Membrane depolarization increases ciliary [Ca(2+)], but only marginally alters cilia beating and cilia-driven fluid velocity within short (~1 min) time frames. We conclude that beating of ependymal motile cilia is not tightly regulated by voltage-gated calcium channels, unlike that of well-studied motile cilia and flagella in protists, such as Paramecia and Chlamydomonas. DOI: http://dx.doi.org/10.7554/eLife.11066.001 eLife Sciences Publications, Ltd 2015-12-09 /pmc/articles/PMC4714969/ /pubmed/26650848 http://dx.doi.org/10.7554/eLife.11066 Text en © 2015, Doerner et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Biophysics and Structural Biology
Doerner, Julia F
Delling, Markus
Clapham, David E
Ion channels and calcium signaling in motile cilia
title Ion channels and calcium signaling in motile cilia
title_full Ion channels and calcium signaling in motile cilia
title_fullStr Ion channels and calcium signaling in motile cilia
title_full_unstemmed Ion channels and calcium signaling in motile cilia
title_short Ion channels and calcium signaling in motile cilia
title_sort ion channels and calcium signaling in motile cilia
topic Biophysics and Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714969/
https://www.ncbi.nlm.nih.gov/pubmed/26650848
http://dx.doi.org/10.7554/eLife.11066
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