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Ion channels and calcium signaling in motile cilia
The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714969/ https://www.ncbi.nlm.nih.gov/pubmed/26650848 http://dx.doi.org/10.7554/eLife.11066 |
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author | Doerner, Julia F Delling, Markus Clapham, David E |
author_facet | Doerner, Julia F Delling, Markus Clapham, David E |
author_sort | Doerner, Julia F |
collection | PubMed |
description | The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration ([Ca(2+)] ~170 nM) is only slightly elevated over cytoplasmic [Ca(2+)] (~100 nM) at steady state. Ca(2+) changes that arise in the cytoplasm rapidly equilibrate in motile cilia. We measured Ca(V)1 voltage-gated calcium channels in ependymal cells, but these channels are not specifically enriched in motile cilia. Membrane depolarization increases ciliary [Ca(2+)], but only marginally alters cilia beating and cilia-driven fluid velocity within short (~1 min) time frames. We conclude that beating of ependymal motile cilia is not tightly regulated by voltage-gated calcium channels, unlike that of well-studied motile cilia and flagella in protists, such as Paramecia and Chlamydomonas. DOI: http://dx.doi.org/10.7554/eLife.11066.001 |
format | Online Article Text |
id | pubmed-4714969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47149692016-01-19 Ion channels and calcium signaling in motile cilia Doerner, Julia F Delling, Markus Clapham, David E eLife Biophysics and Structural Biology The beating of motile cilia generates fluid flow over epithelia in brain ventricles, airways, and Fallopian tubes. Here, we patch clamp single motile cilia of mammalian ependymal cells and examine their potential function as a calcium signaling compartment. Resting motile cilia calcium concentration ([Ca(2+)] ~170 nM) is only slightly elevated over cytoplasmic [Ca(2+)] (~100 nM) at steady state. Ca(2+) changes that arise in the cytoplasm rapidly equilibrate in motile cilia. We measured Ca(V)1 voltage-gated calcium channels in ependymal cells, but these channels are not specifically enriched in motile cilia. Membrane depolarization increases ciliary [Ca(2+)], but only marginally alters cilia beating and cilia-driven fluid velocity within short (~1 min) time frames. We conclude that beating of ependymal motile cilia is not tightly regulated by voltage-gated calcium channels, unlike that of well-studied motile cilia and flagella in protists, such as Paramecia and Chlamydomonas. DOI: http://dx.doi.org/10.7554/eLife.11066.001 eLife Sciences Publications, Ltd 2015-12-09 /pmc/articles/PMC4714969/ /pubmed/26650848 http://dx.doi.org/10.7554/eLife.11066 Text en © 2015, Doerner et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Biophysics and Structural Biology Doerner, Julia F Delling, Markus Clapham, David E Ion channels and calcium signaling in motile cilia |
title | Ion channels and calcium signaling in motile cilia |
title_full | Ion channels and calcium signaling in motile cilia |
title_fullStr | Ion channels and calcium signaling in motile cilia |
title_full_unstemmed | Ion channels and calcium signaling in motile cilia |
title_short | Ion channels and calcium signaling in motile cilia |
title_sort | ion channels and calcium signaling in motile cilia |
topic | Biophysics and Structural Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4714969/ https://www.ncbi.nlm.nih.gov/pubmed/26650848 http://dx.doi.org/10.7554/eLife.11066 |
work_keys_str_mv | AT doernerjuliaf ionchannelsandcalciumsignalinginmotilecilia AT dellingmarkus ionchannelsandcalciumsignalinginmotilecilia AT claphamdavide ionchannelsandcalciumsignalinginmotilecilia |