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The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men
Background: Human papilloma virus (HPV) infection is one of the most common sexually transmitted diseases that affects men like women and infected cutaneous and mucosal squamous epithelium. The aim of the present study was to determine the prevalence of HPV in the semen of oligospermic, azoospermic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iran University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715409/ https://www.ncbi.nlm.nih.gov/pubmed/26793663 |
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author | Nasseri, Sherko Monavari, Seyed Hamidreza Keyvani, Hossein Nikkhoo, Bahram Vahabpour Roudsari, Rouhollah Khazeni, Mohammad |
author_facet | Nasseri, Sherko Monavari, Seyed Hamidreza Keyvani, Hossein Nikkhoo, Bahram Vahabpour Roudsari, Rouhollah Khazeni, Mohammad |
author_sort | Nasseri, Sherko |
collection | PubMed |
description | Background: Human papilloma virus (HPV) infection is one of the most common sexually transmitted diseases that affects men like women and infected cutaneous and mucosal squamous epithelium. The aim of the present study was to determine the prevalence of HPV in the semen of oligospermic, azoospermic and normal patients. Methods: From June 2012 to June 2013, a total of 90 individuals were enrolled in this cross sectional comparative study. The participants were classified into three groups (oligospermia, azoosprmia and normal). This classification was based on a new WHO reference values for human semen characteristics published on 2010. After extraction of DNA from specimens L1 gene of HPV was amplified by nested polymerase chain reaction (Nested-PCR) and the PCR products of positive specimens were genotyped using INNO-LiPA HPV Genotyping Extra assay. Results: Among 50 confirmed oligospermic male, 15 were HPV DNA positive (30%). In azoospemic group we had 8 HPV DNA positive (40%) and in normal group just 3 of 20(15%) samples were positive. Statistical assessment was done with SPSS v.15. Chi-square test showed no significant relationship between 3 groups results. Based on independent samples t-test, we found statistical significant relationship for sperm count (p<0.05) and sperm motility (slow) (p<0.05) in oligospermic group positive samples compared with negative. In the present study, 13 HPV genotypes were detected among positive samples. HPV genotypes 16, 45 in the high risk group and 6,11,42 in the low risk group were more frequent than the others. Conclusion: The current study shows that HPV infection can affect on sperm count and motility and decrease count of sperm cell and decrease motility capability of these cells. |
format | Online Article Text |
id | pubmed-4715409 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Iran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-47154092016-01-20 The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men Nasseri, Sherko Monavari, Seyed Hamidreza Keyvani, Hossein Nikkhoo, Bahram Vahabpour Roudsari, Rouhollah Khazeni, Mohammad Med J Islam Repub Iran Original Article Background: Human papilloma virus (HPV) infection is one of the most common sexually transmitted diseases that affects men like women and infected cutaneous and mucosal squamous epithelium. The aim of the present study was to determine the prevalence of HPV in the semen of oligospermic, azoospermic and normal patients. Methods: From June 2012 to June 2013, a total of 90 individuals were enrolled in this cross sectional comparative study. The participants were classified into three groups (oligospermia, azoosprmia and normal). This classification was based on a new WHO reference values for human semen characteristics published on 2010. After extraction of DNA from specimens L1 gene of HPV was amplified by nested polymerase chain reaction (Nested-PCR) and the PCR products of positive specimens were genotyped using INNO-LiPA HPV Genotyping Extra assay. Results: Among 50 confirmed oligospermic male, 15 were HPV DNA positive (30%). In azoospemic group we had 8 HPV DNA positive (40%) and in normal group just 3 of 20(15%) samples were positive. Statistical assessment was done with SPSS v.15. Chi-square test showed no significant relationship between 3 groups results. Based on independent samples t-test, we found statistical significant relationship for sperm count (p<0.05) and sperm motility (slow) (p<0.05) in oligospermic group positive samples compared with negative. In the present study, 13 HPV genotypes were detected among positive samples. HPV genotypes 16, 45 in the high risk group and 6,11,42 in the low risk group were more frequent than the others. Conclusion: The current study shows that HPV infection can affect on sperm count and motility and decrease count of sperm cell and decrease motility capability of these cells. Iran University of Medical Sciences 2015-10-04 /pmc/articles/PMC4715409/ /pubmed/26793663 Text en © 2015 Iran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0), which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Nasseri, Sherko Monavari, Seyed Hamidreza Keyvani, Hossein Nikkhoo, Bahram Vahabpour Roudsari, Rouhollah Khazeni, Mohammad The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title | The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title_full | The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title_fullStr | The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title_full_unstemmed | The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title_short | The prevalence of Human Papilloma Virus (HPV) infection in the oligospermic and azoospermic men |
title_sort | prevalence of human papilloma virus (hpv) infection in the oligospermic and azoospermic men |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715409/ https://www.ncbi.nlm.nih.gov/pubmed/26793663 |
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