Cargando…

Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria

A ferredoxin:NAD(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes. Since these genes are present in the genomes of many bacteria, we tested for ferredoxin:NAD(+) oxi...

Descripción completa

Detalles Bibliográficos
Autores principales: Hess, Verena, Gallegos, Rene, Jones, J Andrew, Barquera, Blanca, Malamy, Michael H, Müller, Volker
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715464/
https://www.ncbi.nlm.nih.gov/pubmed/26793417
http://dx.doi.org/10.7717/peerj.1515
_version_ 1782410481520082944
author Hess, Verena
Gallegos, Rene
Jones, J Andrew
Barquera, Blanca
Malamy, Michael H
Müller, Volker
author_facet Hess, Verena
Gallegos, Rene
Jones, J Andrew
Barquera, Blanca
Malamy, Michael H
Müller, Volker
author_sort Hess, Verena
collection PubMed
description A ferredoxin:NAD(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes. Since these genes are present in the genomes of many bacteria, we tested for ferredoxin:NAD(+) oxidoreductase activity in cytoplasmic membranes from several different Gram-positive and Gram-negative bacteria that have annotated rnf genes. We found this activity in Clostridium tetanomorphum, Clostridium ljungdahlii, Bacteroides fragilis, and Vibrio cholerae but not in Escherichia coli and Rhodobacter capsulatus. As in A. woodii, the activity was Na(+)-dependent in C. tetanomorphum and B. fragilis but Na(+)-independent in C. ljungdahlii and V. cholerae. We deleted the rnf genes from B. fragilis and demonstrated that the mutant has greatly reduced ferredoxin:NAD(+) oxidoreductase activity. This is the first genetic proof that the rnf genes indeed encode the reduced ferredoxin:NAD(+) oxidoreductase activity.
format Online
Article
Text
id pubmed-4715464
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-47154642016-01-20 Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria Hess, Verena Gallegos, Rene Jones, J Andrew Barquera, Blanca Malamy, Michael H Müller, Volker PeerJ Microbiology A ferredoxin:NAD(+) oxidoreductase was recently discovered as a redox-driven ion pump in the anaerobic, acetogenic bacterium Acetobacterium woodii. The enzyme is assumed to be encoded by the rnf genes. Since these genes are present in the genomes of many bacteria, we tested for ferredoxin:NAD(+) oxidoreductase activity in cytoplasmic membranes from several different Gram-positive and Gram-negative bacteria that have annotated rnf genes. We found this activity in Clostridium tetanomorphum, Clostridium ljungdahlii, Bacteroides fragilis, and Vibrio cholerae but not in Escherichia coli and Rhodobacter capsulatus. As in A. woodii, the activity was Na(+)-dependent in C. tetanomorphum and B. fragilis but Na(+)-independent in C. ljungdahlii and V. cholerae. We deleted the rnf genes from B. fragilis and demonstrated that the mutant has greatly reduced ferredoxin:NAD(+) oxidoreductase activity. This is the first genetic proof that the rnf genes indeed encode the reduced ferredoxin:NAD(+) oxidoreductase activity. PeerJ Inc. 2016-01-11 /pmc/articles/PMC4715464/ /pubmed/26793417 http://dx.doi.org/10.7717/peerj.1515 Text en © 2016 Hess et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Microbiology
Hess, Verena
Gallegos, Rene
Jones, J Andrew
Barquera, Blanca
Malamy, Michael H
Müller, Volker
Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title_full Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title_fullStr Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title_full_unstemmed Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title_short Occurrence of ferredoxin:NAD(+) oxidoreductase activity and its ion specificity in several Gram-positive and Gram-negative bacteria
title_sort occurrence of ferredoxin:nad(+) oxidoreductase activity and its ion specificity in several gram-positive and gram-negative bacteria
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4715464/
https://www.ncbi.nlm.nih.gov/pubmed/26793417
http://dx.doi.org/10.7717/peerj.1515
work_keys_str_mv AT hessverena occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria
AT gallegosrene occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria
AT jonesjandrew occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria
AT barquerablanca occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria
AT malamymichaelh occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria
AT mullervolker occurrenceofferredoxinnadoxidoreductaseactivityanditsionspecificityinseveralgrampositiveandgramnegativebacteria