Cargando…
Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway
OBJECTIVE: This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). MATERIAL AND METHODS: The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (P...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Faculdade de Odontologia de Bauru da Universidade de São Paulo
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716692/ https://www.ncbi.nlm.nih.gov/pubmed/26814456 http://dx.doi.org/10.1590/1678-775720150156 |
_version_ | 1782410569834299392 |
---|---|
author | Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe |
author_facet | Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe |
author_sort | Jianru, YI |
collection | PubMed |
description | OBJECTIVE: This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). MATERIAL AND METHODS: The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm(2) for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. RESULTS: After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. CONCLUSIONS: Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. |
format | Online Article Text |
id | pubmed-4716692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Faculdade de Odontologia de Bauru da Universidade de São Paulo |
record_format | MEDLINE/PubMed |
spelling | pubmed-47166922016-01-20 Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe J Appl Oral Sci Original Articles OBJECTIVE: This study aimed to investigate the potential role of CAMK II pathway in the compression-regulated OPG expression in periodontal ligament cells (PDLCs). MATERIAL AND METHODS: The PDL tissue model was developed by 3-D culturing human PDLCs in a thin sheet of poly lactic-co-glycolic acid (PLGA) scaffolds, which was subjected to static compression of 25 g/cm(2) for 3, 6 and 12 h, with or without treatment of KN-93. After that, the expression of OPG, RANKL and NFATC2 was investigated through real-time PCR and western blot analysis. RESULTS: After static compression, the NFATC2 and RANKL expression was significantly up-regulated, while partially suppressed by KN-93 for 6 and 12 h respectively. The OPG expression was significantly down-regulated by compression in 3 h, started to elevate in 6 h, and significantly up-regulated in 12 h. The up-regulation after 12 h was significantly suppressed by KN-93. CONCLUSIONS: Long-term static compression increases OPG expression in PDLCs, at least partially, via the CAMK II pathway. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2015 /pmc/articles/PMC4716692/ /pubmed/26814456 http://dx.doi.org/10.1590/1678-775720150156 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Jianru, YI MeiLe, LI YANG, Yan ZHENG, Wei Yu, LI ZHAO, Zhihe Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title | Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_full | Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_fullStr | Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_full_unstemmed | Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_short | Static compression regulates OPG expression in periodontal ligament cells via the CAMK II pathway |
title_sort | static compression regulates opg expression in periodontal ligament cells via the camk ii pathway |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716692/ https://www.ncbi.nlm.nih.gov/pubmed/26814456 http://dx.doi.org/10.1590/1678-775720150156 |
work_keys_str_mv | AT jianruyi staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway AT meileli staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway AT yangyan staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway AT zhengwei staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway AT yuli staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway AT zhaozhihe staticcompressionregulatesopgexpressioninperiodontalligamentcellsviathecamkiipathway |