Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)

PREMISE OF THE STUDY: Polymorphic microRNA (miRNA)–based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae). METHODS AND RESULTS: A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Xiaolei, Gui, Songtao, Pan, Lei, Hu, Jihong, Ding, Yi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Botanical Society of America 2016
Materias:
Primer Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716780/
https://www.ncbi.nlm.nih.gov/pubmed/26819861
http://dx.doi.org/10.3732/apps.1500091
_version_ 1782410587812134912
author Wang, Xiaolei
Gui, Songtao
Pan, Lei
Hu, Jihong
Ding, Yi
author_facet Wang, Xiaolei
Gui, Songtao
Pan, Lei
Hu, Jihong
Ding, Yi
author_sort Wang, Xiaolei
collection PubMed
description PREMISE OF THE STUDY: Polymorphic microRNA (miRNA)–based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae). METHODS AND RESULTS: A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences of N. nucifera. After several rounds of screening, 21 primer pairs flanking di-, tri-, or pentanucleotide repeats were identified that revealed high levels of genetic diversity in four populations with two to five alleles per locus. The observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.803, respectively. CONCLUSIONS: The polymorphic microsatellite markers will be useful for studying the genetic diversity and population structure of N. nucifera.
format Online
Article
Text
id pubmed-4716780
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Botanical Society of America
record_format MEDLINE/PubMed
spelling pubmed-47167802016-01-27 Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1) Wang, Xiaolei Gui, Songtao Pan, Lei Hu, Jihong Ding, Yi Appl Plant Sci Primer Note PREMISE OF THE STUDY: Polymorphic microRNA (miRNA)–based microsatellite markers were developed to investigate the genetic diversity and population structure of Nelumbo nucifera (Nelumbonaceae). METHODS AND RESULTS: A total of 485 miRNA-based microsatellites were found from the genomic DNA sequences of N. nucifera. After several rounds of screening, 21 primer pairs flanking di-, tri-, or pentanucleotide repeats were identified that revealed high levels of genetic diversity in four populations with two to five alleles per locus. The observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.803, respectively. CONCLUSIONS: The polymorphic microsatellite markers will be useful for studying the genetic diversity and population structure of N. nucifera. Botanical Society of America 2016-01-06 /pmc/articles/PMC4716780/ /pubmed/26819861 http://dx.doi.org/10.3732/apps.1500091 Text en © 2016 Wang et al. Published by the Botanical Society of America http://creativecommons.org/licenses/by-nc/4.0/ This work is licensed under a Creative Commons Attribution License (CC-BY-NC-SA).
spellingShingle Primer Note
Wang, Xiaolei
Gui, Songtao
Pan, Lei
Hu, Jihong
Ding, Yi
Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title_full Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title_fullStr Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title_full_unstemmed Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title_short Development and characterization of polymorphic microRNA-based microsatellite markers in Nelumbo nucifera (Nelumbonaceae)(1)
title_sort development and characterization of polymorphic microrna-based microsatellite markers in nelumbo nucifera (nelumbonaceae)(1)
topic Primer Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716780/
https://www.ncbi.nlm.nih.gov/pubmed/26819861
http://dx.doi.org/10.3732/apps.1500091
work_keys_str_mv AT wangxiaolei developmentandcharacterizationofpolymorphicmicrornabasedmicrosatellitemarkersinnelumbonuciferanelumbonaceae1
AT guisongtao developmentandcharacterizationofpolymorphicmicrornabasedmicrosatellitemarkersinnelumbonuciferanelumbonaceae1
AT panlei developmentandcharacterizationofpolymorphicmicrornabasedmicrosatellitemarkersinnelumbonuciferanelumbonaceae1
AT hujihong developmentandcharacterizationofpolymorphicmicrornabasedmicrosatellitemarkersinnelumbonuciferanelumbonaceae1
AT dingyi developmentandcharacterizationofpolymorphicmicrornabasedmicrosatellitemarkersinnelumbonuciferanelumbonaceae1

Ejemplares similares