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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through dif...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716842/ https://www.ncbi.nlm.nih.gov/pubmed/26457503 http://dx.doi.org/10.7554/eLife.10501 |
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author | Tu, Kimberly C Cheng, Li-Chun TK Vu, Hanh Lange, Jeffrey J McKinney, Sean A Seidel, Chris W Sánchez Alvarado, Alejandro |
author_facet | Tu, Kimberly C Cheng, Li-Chun TK Vu, Hanh Lange, Jeffrey J McKinney, Sean A Seidel, Chris W Sánchez Alvarado, Alejandro |
author_sort | Tu, Kimberly C |
collection | PubMed |
description | Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through differentiation pathways to produce specific cell types. The planarian epidermis is a simple tissue that undergoes rapid turnover. We found that as epidermal progeny differentiate, they progress through multiple spatiotemporal transition states with distinct gene expression profiles. We also identified a conserved early growth response family transcription factor, egr-5, that is essential for epidermal differentiation. Disruption of epidermal integrity by egr-5 RNAi triggers a global stress response that induces the proliferation of neoblasts and the concomitant expansion of not only epidermal, but also multiple progenitor cell populations. Our results further establish the planarian epidermis as a novel paradigm to uncover the molecular mechanisms regulating ASC specification in vivo. DOI: http://dx.doi.org/10.7554/eLife.10501.001 |
format | Online Article Text |
id | pubmed-4716842 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47168422016-03-17 Egr-5 is a post-mitotic regulator of planarian epidermal differentiation Tu, Kimberly C Cheng, Li-Chun TK Vu, Hanh Lange, Jeffrey J McKinney, Sean A Seidel, Chris W Sánchez Alvarado, Alejandro eLife Cell Biology Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through differentiation pathways to produce specific cell types. The planarian epidermis is a simple tissue that undergoes rapid turnover. We found that as epidermal progeny differentiate, they progress through multiple spatiotemporal transition states with distinct gene expression profiles. We also identified a conserved early growth response family transcription factor, egr-5, that is essential for epidermal differentiation. Disruption of epidermal integrity by egr-5 RNAi triggers a global stress response that induces the proliferation of neoblasts and the concomitant expansion of not only epidermal, but also multiple progenitor cell populations. Our results further establish the planarian epidermis as a novel paradigm to uncover the molecular mechanisms regulating ASC specification in vivo. DOI: http://dx.doi.org/10.7554/eLife.10501.001 eLife Sciences Publications, Ltd 2015-10-12 /pmc/articles/PMC4716842/ /pubmed/26457503 http://dx.doi.org/10.7554/eLife.10501 Text en © 2015, Tu et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Cell Biology Tu, Kimberly C Cheng, Li-Chun TK Vu, Hanh Lange, Jeffrey J McKinney, Sean A Seidel, Chris W Sánchez Alvarado, Alejandro Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title_full | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title_fullStr | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title_full_unstemmed | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title_short | Egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
title_sort | egr-5 is a post-mitotic regulator of planarian epidermal differentiation |
topic | Cell Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716842/ https://www.ncbi.nlm.nih.gov/pubmed/26457503 http://dx.doi.org/10.7554/eLife.10501 |
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