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Egr-5 is a post-mitotic regulator of planarian epidermal differentiation

Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through dif...

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Autores principales: Tu, Kimberly C, Cheng, Li-Chun, TK Vu, Hanh, Lange, Jeffrey J, McKinney, Sean A, Seidel, Chris W, Sánchez Alvarado, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716842/
https://www.ncbi.nlm.nih.gov/pubmed/26457503
http://dx.doi.org/10.7554/eLife.10501
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author Tu, Kimberly C
Cheng, Li-Chun
TK Vu, Hanh
Lange, Jeffrey J
McKinney, Sean A
Seidel, Chris W
Sánchez Alvarado, Alejandro
author_facet Tu, Kimberly C
Cheng, Li-Chun
TK Vu, Hanh
Lange, Jeffrey J
McKinney, Sean A
Seidel, Chris W
Sánchez Alvarado, Alejandro
author_sort Tu, Kimberly C
collection PubMed
description Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through differentiation pathways to produce specific cell types. The planarian epidermis is a simple tissue that undergoes rapid turnover. We found that as epidermal progeny differentiate, they progress through multiple spatiotemporal transition states with distinct gene expression profiles. We also identified a conserved early growth response family transcription factor, egr-5, that is essential for epidermal differentiation. Disruption of epidermal integrity by egr-5 RNAi triggers a global stress response that induces the proliferation of neoblasts and the concomitant expansion of not only epidermal, but also multiple progenitor cell populations. Our results further establish the planarian epidermis as a novel paradigm to uncover the molecular mechanisms regulating ASC specification in vivo. DOI: http://dx.doi.org/10.7554/eLife.10501.001
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spelling pubmed-47168422016-03-17 Egr-5 is a post-mitotic regulator of planarian epidermal differentiation Tu, Kimberly C Cheng, Li-Chun TK Vu, Hanh Lange, Jeffrey J McKinney, Sean A Seidel, Chris W Sánchez Alvarado, Alejandro eLife Cell Biology Neoblasts are an abundant, heterogeneous population of adult stem cells (ASCs) that facilitate the maintenance of planarian tissues and organs, providing a powerful system to study ASC self-renewal and differentiation dynamics. It is unknown how the collective output of neoblasts transit through differentiation pathways to produce specific cell types. The planarian epidermis is a simple tissue that undergoes rapid turnover. We found that as epidermal progeny differentiate, they progress through multiple spatiotemporal transition states with distinct gene expression profiles. We also identified a conserved early growth response family transcription factor, egr-5, that is essential for epidermal differentiation. Disruption of epidermal integrity by egr-5 RNAi triggers a global stress response that induces the proliferation of neoblasts and the concomitant expansion of not only epidermal, but also multiple progenitor cell populations. Our results further establish the planarian epidermis as a novel paradigm to uncover the molecular mechanisms regulating ASC specification in vivo. DOI: http://dx.doi.org/10.7554/eLife.10501.001 eLife Sciences Publications, Ltd 2015-10-12 /pmc/articles/PMC4716842/ /pubmed/26457503 http://dx.doi.org/10.7554/eLife.10501 Text en © 2015, Tu et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Tu, Kimberly C
Cheng, Li-Chun
TK Vu, Hanh
Lange, Jeffrey J
McKinney, Sean A
Seidel, Chris W
Sánchez Alvarado, Alejandro
Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title_full Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title_fullStr Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title_full_unstemmed Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title_short Egr-5 is a post-mitotic regulator of planarian epidermal differentiation
title_sort egr-5 is a post-mitotic regulator of planarian epidermal differentiation
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4716842/
https://www.ncbi.nlm.nih.gov/pubmed/26457503
http://dx.doi.org/10.7554/eLife.10501
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