Cargando…

Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi

Plastids produce a vast diversity of transcripts. These include mature transcripts containing coding sequences, and their processing precursors, as well as transcripts that lack direct coding functions, such as antisense transcripts. Although plastid transcriptomes have been characterised for many p...

Descripción completa

Detalles Bibliográficos
Autores principales: Dorrell, Richard G., Hinksman, George A., Howe, Christopher J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717168/
https://www.ncbi.nlm.nih.gov/pubmed/26768263
http://dx.doi.org/10.1007/s11103-015-0408-9
_version_ 1782410606769340416
author Dorrell, Richard G.
Hinksman, George A.
Howe, Christopher J.
author_facet Dorrell, Richard G.
Hinksman, George A.
Howe, Christopher J.
author_sort Dorrell, Richard G.
collection PubMed
description Plastids produce a vast diversity of transcripts. These include mature transcripts containing coding sequences, and their processing precursors, as well as transcripts that lack direct coding functions, such as antisense transcripts. Although plastid transcriptomes have been characterised for many plant species, less is known about the transcripts produced in other plastid lineages. We characterised the transcripts produced in the fucoxanthin-containing plastids of the dinoflagellate alga Karenia mikimotoi. This plastid lineage, acquired through tertiary endosymbiosis, utilises transcript processing pathways that are very different from those found in plants and green algae, including 3′ poly(U) tail addition, and extensive substitutional editing of transcript sequences. We have sequenced the plastid transcriptome of K. mikimotoi, and have detected evidence for divergent evolution of fucoxanthin plastid genomes. We have additionally characterised polycistronic and monocistronic transcripts from two plastid loci, psbD-tRNA(Met)-ycf4 and rpl36-rps13-rps11. We find evidence for a range of transcripts produced from each locus that differ in terms of editing state, 5′ end cleavage position, and poly(U) tail addition. Finally, we identify antisense transcripts in K. mikimotoi, which appear to undergo different processing events from the corresponding sense transcripts. Overall, our study provides insights into the diversity of transcripts and processing intermediates found in plastid lineages across the eukaryotes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-015-0408-9) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-4717168
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-47171682016-01-25 Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi Dorrell, Richard G. Hinksman, George A. Howe, Christopher J. Plant Mol Biol Article Plastids produce a vast diversity of transcripts. These include mature transcripts containing coding sequences, and their processing precursors, as well as transcripts that lack direct coding functions, such as antisense transcripts. Although plastid transcriptomes have been characterised for many plant species, less is known about the transcripts produced in other plastid lineages. We characterised the transcripts produced in the fucoxanthin-containing plastids of the dinoflagellate alga Karenia mikimotoi. This plastid lineage, acquired through tertiary endosymbiosis, utilises transcript processing pathways that are very different from those found in plants and green algae, including 3′ poly(U) tail addition, and extensive substitutional editing of transcript sequences. We have sequenced the plastid transcriptome of K. mikimotoi, and have detected evidence for divergent evolution of fucoxanthin plastid genomes. We have additionally characterised polycistronic and monocistronic transcripts from two plastid loci, psbD-tRNA(Met)-ycf4 and rpl36-rps13-rps11. We find evidence for a range of transcripts produced from each locus that differ in terms of editing state, 5′ end cleavage position, and poly(U) tail addition. Finally, we identify antisense transcripts in K. mikimotoi, which appear to undergo different processing events from the corresponding sense transcripts. Overall, our study provides insights into the diversity of transcripts and processing intermediates found in plastid lineages across the eukaryotes. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-015-0408-9) contains supplementary material, which is available to authorized users. Springer Netherlands 2016-01-14 2016 /pmc/articles/PMC4717168/ /pubmed/26768263 http://dx.doi.org/10.1007/s11103-015-0408-9 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Dorrell, Richard G.
Hinksman, George A.
Howe, Christopher J.
Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title_full Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title_fullStr Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title_full_unstemmed Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title_short Diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga Karenia mikimotoi
title_sort diversity of transcripts and transcript processing forms in plastids of the dinoflagellate alga karenia mikimotoi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717168/
https://www.ncbi.nlm.nih.gov/pubmed/26768263
http://dx.doi.org/10.1007/s11103-015-0408-9
work_keys_str_mv AT dorrellrichardg diversityoftranscriptsandtranscriptprocessingformsinplastidsofthedinoflagellatealgakareniamikimotoi
AT hinksmangeorgea diversityoftranscriptsandtranscriptprocessingformsinplastidsofthedinoflagellatealgakareniamikimotoi
AT howechristopherj diversityoftranscriptsandtranscriptprocessingformsinplastidsofthedinoflagellatealgakareniamikimotoi