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Modal variety of microsatellite instability in human endometrial carcinomas
PURPOSE: Microsatellite instability (MSI) in human endometrial cancer (EC) was analysed using a unique fluorescent technique. MSI is associated with various human neoplasms. However, the reported frequency of MSI differs widely in each malignancy. Methodological difficulties have in fact been pointe...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717170/ https://www.ncbi.nlm.nih.gov/pubmed/26298837 http://dx.doi.org/10.1007/s00432-015-2030-2 |
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author | Eto, Takako Zhao, Yan Maruyama, Akiko Miyashita, Kaname Yasui, Aiko Nakao, Seiki Taguchi, Kenichi Shimokawa, Mototsugu Oda, Shinya Saito, Toshiaki |
author_facet | Eto, Takako Zhao, Yan Maruyama, Akiko Miyashita, Kaname Yasui, Aiko Nakao, Seiki Taguchi, Kenichi Shimokawa, Mototsugu Oda, Shinya Saito, Toshiaki |
author_sort | Eto, Takako |
collection | PubMed |
description | PURPOSE: Microsatellite instability (MSI) in human endometrial cancer (EC) was analysed using a unique fluorescent technique. MSI is associated with various human neoplasms. However, the reported frequency of MSI differs widely in each malignancy. Methodological difficulties have in fact been pointed out in its assay techniques. METHODS: We previously established a sensitive fluorescent technique in which the major methodological problems are overcome. Application of this technique has revealed two distinct modes of microsatellite alterations, i.e. Type A and Type B. In the present study, we have applied this technique to 94 ECs. RESULTS: Significant microsatellite alterations were observed in 38 (40.4 %) tumours of the panel. The two modes, Type A and Type B, were indeed observed in this malignancy. More importantly, we found that the modes more closely correlated with the molecular and clinicopathological backgrounds of the tumours than the established and widely used MSI grades, MSI-H and MSI-L. Type B MSI widely correlated with family history of hereditary non-polyposis colorectal cancer-associated cancers, whereas MSI-H only did with that of colorectal cancer. Furthermore, mutation in the KRAS oncogene, which has been regarded as generally infrequent in microsatellite-unstable tumours, was clearly associated with Type A MSI. CONCLUSIONS: Our observations may suggest a biological relevance and a potential utility of the modal classification of MSI and, furthermore, added complexities to genomic instability underlying tumourigenesis in human endometrium. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00432-015-2030-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4717170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-47171702016-01-25 Modal variety of microsatellite instability in human endometrial carcinomas Eto, Takako Zhao, Yan Maruyama, Akiko Miyashita, Kaname Yasui, Aiko Nakao, Seiki Taguchi, Kenichi Shimokawa, Mototsugu Oda, Shinya Saito, Toshiaki J Cancer Res Clin Oncol Original Article – Cancer Research PURPOSE: Microsatellite instability (MSI) in human endometrial cancer (EC) was analysed using a unique fluorescent technique. MSI is associated with various human neoplasms. However, the reported frequency of MSI differs widely in each malignancy. Methodological difficulties have in fact been pointed out in its assay techniques. METHODS: We previously established a sensitive fluorescent technique in which the major methodological problems are overcome. Application of this technique has revealed two distinct modes of microsatellite alterations, i.e. Type A and Type B. In the present study, we have applied this technique to 94 ECs. RESULTS: Significant microsatellite alterations were observed in 38 (40.4 %) tumours of the panel. The two modes, Type A and Type B, were indeed observed in this malignancy. More importantly, we found that the modes more closely correlated with the molecular and clinicopathological backgrounds of the tumours than the established and widely used MSI grades, MSI-H and MSI-L. Type B MSI widely correlated with family history of hereditary non-polyposis colorectal cancer-associated cancers, whereas MSI-H only did with that of colorectal cancer. Furthermore, mutation in the KRAS oncogene, which has been regarded as generally infrequent in microsatellite-unstable tumours, was clearly associated with Type A MSI. CONCLUSIONS: Our observations may suggest a biological relevance and a potential utility of the modal classification of MSI and, furthermore, added complexities to genomic instability underlying tumourigenesis in human endometrium. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00432-015-2030-2) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-08-23 2016 /pmc/articles/PMC4717170/ /pubmed/26298837 http://dx.doi.org/10.1007/s00432-015-2030-2 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Article – Cancer Research Eto, Takako Zhao, Yan Maruyama, Akiko Miyashita, Kaname Yasui, Aiko Nakao, Seiki Taguchi, Kenichi Shimokawa, Mototsugu Oda, Shinya Saito, Toshiaki Modal variety of microsatellite instability in human endometrial carcinomas |
title | Modal variety of microsatellite instability in human endometrial carcinomas |
title_full | Modal variety of microsatellite instability in human endometrial carcinomas |
title_fullStr | Modal variety of microsatellite instability in human endometrial carcinomas |
title_full_unstemmed | Modal variety of microsatellite instability in human endometrial carcinomas |
title_short | Modal variety of microsatellite instability in human endometrial carcinomas |
title_sort | modal variety of microsatellite instability in human endometrial carcinomas |
topic | Original Article – Cancer Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717170/ https://www.ncbi.nlm.nih.gov/pubmed/26298837 http://dx.doi.org/10.1007/s00432-015-2030-2 |
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