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Immune Regulation of Interleukin-27 in Malignant Pleural Effusion

BACKGROUND: Interleukin (IL)-27 has been reported to have anti-proliferate and anti-angiogenic activities on cancer cells. However, the involvement of IL-27 in malignant pleural effusion (MPE) remains unknown. Thus, in this research, we compared the immune functions of IL-27, interferon (IFN)-γ, and...

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Autores principales: Li, Shi, You, Wen-Jie, Zhang, Jian-Chu, Zhou, Qiong, Shi, Huan-Zhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717932/
https://www.ncbi.nlm.nih.gov/pubmed/26168835
http://dx.doi.org/10.4103/0366-6999.160556
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author Li, Shi
You, Wen-Jie
Zhang, Jian-Chu
Zhou, Qiong
Shi, Huan-Zhong
author_facet Li, Shi
You, Wen-Jie
Zhang, Jian-Chu
Zhou, Qiong
Shi, Huan-Zhong
author_sort Li, Shi
collection PubMed
description BACKGROUND: Interleukin (IL)-27 has been reported to have anti-proliferate and anti-angiogenic activities on cancer cells. However, the involvement of IL-27 in malignant pleural effusion (MPE) remains unknown. Thus, in this research, we compared the immune functions of IL-27, interferon (IFN)-γ, and IL-17 on lung cancer cells and revealed the regulatory mechanism of IL-27 in MPE. METHODS: The distribution of IL-27 in both MPE and blood was evaluated by enzyme-linked immunosorbent assay and flow cytometry. The expressions of cytokine receptors and the levels of the phosphorylated signal transducer and activator of transcription (STAT) signalings were detected by flow cytometry. As well as the effects of proliferation, apoptosis, migration, and adherent activity of IL-27, IFN-γ, and IL-17 on lung cancer cells were also explored. RESULTS: The expression of IL-27 was increased in MPE when compared with blood (147.3 ± 25.1 pg/ml vs. 100.3 ± 13.9 pg/ml, P = 0.04). IL-27 was noted to suppress both proliferation (18.33 ± 0.21 vs. 27.77 ± 0.88, P = 0.0005) and migration (1.82 ± 0.44 vs. 3.13 ± 0.07, P = 0.04) of A549 cells, but obviously promoted apoptosis of A549 cells (9.47 ± 1.14 vs. 4.96 ± 0.17, P = 0.02) by activating STAT1 signaling. Interestingly, IL-27 played totally opposite effects on A549 cells by activating STAT3 pathway. Moreover, IL-27 exerted different intercellular adherent activities of A549 cells to pleural mesothelial cell monolayer by activating different STAT signalings. CONCLUSIONS: IL-27 might exert an important immune regulation on lung cancer cells in human pleural malignant environment.
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spelling pubmed-47179322016-04-04 Immune Regulation of Interleukin-27 in Malignant Pleural Effusion Li, Shi You, Wen-Jie Zhang, Jian-Chu Zhou, Qiong Shi, Huan-Zhong Chin Med J (Engl) Original Article BACKGROUND: Interleukin (IL)-27 has been reported to have anti-proliferate and anti-angiogenic activities on cancer cells. However, the involvement of IL-27 in malignant pleural effusion (MPE) remains unknown. Thus, in this research, we compared the immune functions of IL-27, interferon (IFN)-γ, and IL-17 on lung cancer cells and revealed the regulatory mechanism of IL-27 in MPE. METHODS: The distribution of IL-27 in both MPE and blood was evaluated by enzyme-linked immunosorbent assay and flow cytometry. The expressions of cytokine receptors and the levels of the phosphorylated signal transducer and activator of transcription (STAT) signalings were detected by flow cytometry. As well as the effects of proliferation, apoptosis, migration, and adherent activity of IL-27, IFN-γ, and IL-17 on lung cancer cells were also explored. RESULTS: The expression of IL-27 was increased in MPE when compared with blood (147.3 ± 25.1 pg/ml vs. 100.3 ± 13.9 pg/ml, P = 0.04). IL-27 was noted to suppress both proliferation (18.33 ± 0.21 vs. 27.77 ± 0.88, P = 0.0005) and migration (1.82 ± 0.44 vs. 3.13 ± 0.07, P = 0.04) of A549 cells, but obviously promoted apoptosis of A549 cells (9.47 ± 1.14 vs. 4.96 ± 0.17, P = 0.02) by activating STAT1 signaling. Interestingly, IL-27 played totally opposite effects on A549 cells by activating STAT3 pathway. Moreover, IL-27 exerted different intercellular adherent activities of A549 cells to pleural mesothelial cell monolayer by activating different STAT signalings. CONCLUSIONS: IL-27 might exert an important immune regulation on lung cancer cells in human pleural malignant environment. Medknow Publications & Media Pvt Ltd 2015-07-20 /pmc/articles/PMC4717932/ /pubmed/26168835 http://dx.doi.org/10.4103/0366-6999.160556 Text en Copyright: © 2015 Chinese Medical Journal http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Original Article
Li, Shi
You, Wen-Jie
Zhang, Jian-Chu
Zhou, Qiong
Shi, Huan-Zhong
Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title_full Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title_fullStr Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title_full_unstemmed Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title_short Immune Regulation of Interleukin-27 in Malignant Pleural Effusion
title_sort immune regulation of interleukin-27 in malignant pleural effusion
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4717932/
https://www.ncbi.nlm.nih.gov/pubmed/26168835
http://dx.doi.org/10.4103/0366-6999.160556
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