Cargando…

A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium

INTRODUCTION. A cross-sectional study was undertaken to determine the prevalence of Giardia and Cryptosporidium in calves of Palermo area (Sicily) and to evaluate the occupational risk associated with occurrence of zoonotic genotypes. METHODS. A total of 217 faecal samples, from 149 calves (between...

Descripción completa

Detalles Bibliográficos
Autores principales: DI PIAZZA, F., DI BENEDETTO, M.A., MAIDA, C.M., GLORIOSO, S., ADAMO, G., MAZZOLA, T., FIRENZE, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pacini Editore SpA 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718323/
https://www.ncbi.nlm.nih.gov/pubmed/24779283
_version_ 1782410779474001920
author DI PIAZZA, F.
DI BENEDETTO, M.A.
MAIDA, C.M.
GLORIOSO, S.
ADAMO, G.
MAZZOLA, T.
FIRENZE, A.
author_facet DI PIAZZA, F.
DI BENEDETTO, M.A.
MAIDA, C.M.
GLORIOSO, S.
ADAMO, G.
MAZZOLA, T.
FIRENZE, A.
author_sort DI PIAZZA, F.
collection PubMed
description INTRODUCTION. A cross-sectional study was undertaken to determine the prevalence of Giardia and Cryptosporidium in calves of Palermo area (Sicily) and to evaluate the occupational risk associated with occurrence of zoonotic genotypes. METHODS. A total of 217 faecal samples, from 149 calves (between 2 and 240 days of age) and 68 farmers, were collected in 19 cattlefarms of Palermo area. A questionnaire regarding demographic characteristics and personal hygienic measures was submitted to all farmers. All faecal samples were analyzed by Immunofluorescence assay and Polimerase Chain Reaction (PCR); genotypes were determined by DNA sequencing of Triose Phosphate Isomerase gene for Giardia and Small Subunit Ribosomal RNA gene for Cryptosporidium. RESULTS. None farmer tested was positive for Giardia and Cryptosporidium, whereas these protozoa were respectively detected in 53 (including 5 with zoonotic G. duodenalis genotype A) and 17 (of which 1 with zoonotic C. ubiquitum) of the examined calves. DISCUSSION. The results indicate that the risk of transmitting both protozoa to farmers in Palermo area is negligible although it cannot be considered null because of identification of human genotypes/ species in calves.
format Online
Article
Text
id pubmed-4718323
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Pacini Editore SpA
record_format MEDLINE/PubMed
spelling pubmed-47183232016-02-02 A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium DI PIAZZA, F. DI BENEDETTO, M.A. MAIDA, C.M. GLORIOSO, S. ADAMO, G. MAZZOLA, T. FIRENZE, A. J Prev Med Hyg Original Article INTRODUCTION. A cross-sectional study was undertaken to determine the prevalence of Giardia and Cryptosporidium in calves of Palermo area (Sicily) and to evaluate the occupational risk associated with occurrence of zoonotic genotypes. METHODS. A total of 217 faecal samples, from 149 calves (between 2 and 240 days of age) and 68 farmers, were collected in 19 cattlefarms of Palermo area. A questionnaire regarding demographic characteristics and personal hygienic measures was submitted to all farmers. All faecal samples were analyzed by Immunofluorescence assay and Polimerase Chain Reaction (PCR); genotypes were determined by DNA sequencing of Triose Phosphate Isomerase gene for Giardia and Small Subunit Ribosomal RNA gene for Cryptosporidium. RESULTS. None farmer tested was positive for Giardia and Cryptosporidium, whereas these protozoa were respectively detected in 53 (including 5 with zoonotic G. duodenalis genotype A) and 17 (of which 1 with zoonotic C. ubiquitum) of the examined calves. DISCUSSION. The results indicate that the risk of transmitting both protozoa to farmers in Palermo area is negligible although it cannot be considered null because of identification of human genotypes/ species in calves. Pacini Editore SpA 2013-12 /pmc/articles/PMC4718323/ /pubmed/24779283 Text en © Copyright by Pacini Editore SpA, Pisa, Italy http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License, which permits for noncommercial use, distribution, and reproduction in any digital medium, provided the original work is properly cited and is not altered in any way. For details, please refer to http://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Original Article
DI PIAZZA, F.
DI BENEDETTO, M.A.
MAIDA, C.M.
GLORIOSO, S.
ADAMO, G.
MAZZOLA, T.
FIRENZE, A.
A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title_full A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title_fullStr A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title_full_unstemmed A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title_short A study on occupational exposure of Sicilian farmers to Giardia and Cryptosporidium
title_sort study on occupational exposure of sicilian farmers to giardia and cryptosporidium
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718323/
https://www.ncbi.nlm.nih.gov/pubmed/24779283
work_keys_str_mv AT dipiazzaf astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT dibenedettoma astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT maidacm astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT gloriosos astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT adamog astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT mazzolat astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT firenzea astudyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT dipiazzaf studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT dibenedettoma studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT maidacm studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT gloriosos studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT adamog studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT mazzolat studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium
AT firenzea studyonoccupationalexposureofsicilianfarmerstogiardiaandcryptosporidium