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Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production

The BaM6PI gene encoding a mannose-6-phosphate isomerase (M6PI, EC 5.3.1.8) was cloned from Bacillus amyloliquefaciens DSM7 and overexpressed in Escherichia coli. The enzyme activity of BaM6PI was optimal at pH and temperature of 7.5 and 70°C, respectively, with a k(cat)/K(m) of 13,900 s(-1) mM(-1)...

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Detalles Bibliográficos
Autores principales:	Sigdel, Sujan, Singh, Ranjitha, Kim, Tae-Su, Li, Jinglin, Kim, Sang-Yong, Kim, In-Won, Jung, Woo-Suk, Pan, Cheol-Ho, Kang, Yun Chan, Lee, Jung-Kul
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718643/ https://www.ncbi.nlm.nih.gov/pubmed/26171785 http://dx.doi.org/10.1371/journal.pone.0131585

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