Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila

The evolutionarily conserved Crumbs protein is required for epithelial polarity and morphogenesis. Here we identify a novel role of Crumbs as a negative regulator of actomyosin dynamics during dorsal closure in the Drosophila embryo. Embryos carrying a mutation in the FERM (protein 4.1/ezrin/radixin...

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Autores principales: Flores-Benitez, David, Knust, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718732/
https://www.ncbi.nlm.nih.gov/pubmed/26544546
http://dx.doi.org/10.7554/eLife.07398
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author Flores-Benitez, David
Knust, Elisabeth
author_facet Flores-Benitez, David
Knust, Elisabeth
author_sort Flores-Benitez, David
collection PubMed
description The evolutionarily conserved Crumbs protein is required for epithelial polarity and morphogenesis. Here we identify a novel role of Crumbs as a negative regulator of actomyosin dynamics during dorsal closure in the Drosophila embryo. Embryos carrying a mutation in the FERM (protein 4.1/ezrin/radixin/moesin) domain-binding motif of Crumbs die due to an overactive actomyosin network associated with disrupted adherens junctions. This phenotype is restricted to the amnioserosa and does not affect other embryonic epithelia. This function of Crumbs requires DMoesin, the Rho1-GTPase, class-I p21-activated kinases and the Arp2/3 complex. Data presented here point to a critical role of Crumbs in regulating actomyosin dynamics, cell junctions and morphogenesis. DOI: http://dx.doi.org/10.7554/eLife.07398.001
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spelling pubmed-47187322016-01-21 Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila Flores-Benitez, David Knust, Elisabeth eLife Cell Biology The evolutionarily conserved Crumbs protein is required for epithelial polarity and morphogenesis. Here we identify a novel role of Crumbs as a negative regulator of actomyosin dynamics during dorsal closure in the Drosophila embryo. Embryos carrying a mutation in the FERM (protein 4.1/ezrin/radixin/moesin) domain-binding motif of Crumbs die due to an overactive actomyosin network associated with disrupted adherens junctions. This phenotype is restricted to the amnioserosa and does not affect other embryonic epithelia. This function of Crumbs requires DMoesin, the Rho1-GTPase, class-I p21-activated kinases and the Arp2/3 complex. Data presented here point to a critical role of Crumbs in regulating actomyosin dynamics, cell junctions and morphogenesis. DOI: http://dx.doi.org/10.7554/eLife.07398.001 eLife Sciences Publications, Ltd 2015-11-06 /pmc/articles/PMC4718732/ /pubmed/26544546 http://dx.doi.org/10.7554/eLife.07398 Text en © 2015, Flores-Benitez et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Flores-Benitez, David
Knust, Elisabeth
Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title_full Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title_fullStr Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title_full_unstemmed Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title_short Crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in Drosophila
title_sort crumbs is an essential regulator of cytoskeletal dynamics and cell-cell adhesion during dorsal closure in drosophila
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718732/
https://www.ncbi.nlm.nih.gov/pubmed/26544546
http://dx.doi.org/10.7554/eLife.07398
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AT knustelisabeth crumbsisanessentialregulatorofcytoskeletaldynamicsandcellcelladhesionduringdorsalclosureindrosophila

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