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Temporal transcriptomics suggest that twin-peaking genes reset the clock
The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdisse...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718813/ https://www.ncbi.nlm.nih.gov/pubmed/26523393 http://dx.doi.org/10.7554/eLife.10518 |
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author | Pembroke, William G Babbs, Arran Davies, Kay E Ponting, Chris P Oliver, Peter L |
author_facet | Pembroke, William G Babbs, Arran Davies, Kay E Ponting, Chris P Oliver, Peter L |
author_sort | Pembroke, William G |
collection | PubMed |
description | The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light / dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN. Another group of 766 genes unexpectedly peaked twice, near both the start and end of the dark phase; this twin-peaking group is significantly enriched for synaptic transmission genes that are crucial for light-induced phase shifting of the circadian clock. 341 intergenic non-coding RNAs, together with novel exons of annotated protein-coding genes, including Cry1, also show specific circadian expression variation. Overall, our data provide an important chronobiological resource (www.wgpembroke.com/shiny/SCNseq/) and allow us to propose that transcriptional timing in the SCN is gating clock resetting mechanisms. DOI: http://dx.doi.org/10.7554/eLife.10518.001 |
format | Online Article Text |
id | pubmed-4718813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47188132016-01-21 Temporal transcriptomics suggest that twin-peaking genes reset the clock Pembroke, William G Babbs, Arran Davies, Kay E Ponting, Chris P Oliver, Peter L eLife Genomics and Evolutionary Biology The mammalian suprachiasmatic nucleus (SCN) drives daily rhythmic behavior and physiology, yet a detailed understanding of its coordinated transcriptional programmes is lacking. To reveal the finer details of circadian variation in the mammalian SCN transcriptome we combined laser-capture microdissection (LCM) and RNA-seq over a 24 hr light / dark cycle. We show that 7-times more genes exhibited a classic sinusoidal expression signature than previously observed in the SCN. Another group of 766 genes unexpectedly peaked twice, near both the start and end of the dark phase; this twin-peaking group is significantly enriched for synaptic transmission genes that are crucial for light-induced phase shifting of the circadian clock. 341 intergenic non-coding RNAs, together with novel exons of annotated protein-coding genes, including Cry1, also show specific circadian expression variation. Overall, our data provide an important chronobiological resource (www.wgpembroke.com/shiny/SCNseq/) and allow us to propose that transcriptional timing in the SCN is gating clock resetting mechanisms. DOI: http://dx.doi.org/10.7554/eLife.10518.001 eLife Sciences Publications, Ltd 2015-11-02 /pmc/articles/PMC4718813/ /pubmed/26523393 http://dx.doi.org/10.7554/eLife.10518 Text en © 2015, Pembroke et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Genomics and Evolutionary Biology Pembroke, William G Babbs, Arran Davies, Kay E Ponting, Chris P Oliver, Peter L Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title | Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title_full | Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title_fullStr | Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title_full_unstemmed | Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title_short | Temporal transcriptomics suggest that twin-peaking genes reset the clock |
title_sort | temporal transcriptomics suggest that twin-peaking genes reset the clock |
topic | Genomics and Evolutionary Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4718813/ https://www.ncbi.nlm.nih.gov/pubmed/26523393 http://dx.doi.org/10.7554/eLife.10518 |
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