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Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal

Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid...

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Autores principales: Delfosse, Kathleen, Wozny, Michael R., Jaipargas, Erica-Ashley, Barton, Kiah A., Anderson, Cole, Mathur, Jaideep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719081/
https://www.ncbi.nlm.nih.gov/pubmed/26834765
http://dx.doi.org/10.3389/fpls.2015.01253
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author Delfosse, Kathleen
Wozny, Michael R.
Jaipargas, Erica-Ashley
Barton, Kiah A.
Anderson, Cole
Mathur, Jaideep
author_facet Delfosse, Kathleen
Wozny, Michael R.
Jaipargas, Erica-Ashley
Barton, Kiah A.
Anderson, Cole
Mathur, Jaideep
author_sort Delfosse, Kathleen
collection PubMed
description Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid envelope, nucleoids, plastoglobuli, and starch grains have been efficiently highlighted in living plant cells. In addition, stroma filled membrane extensions called stromules have drawn attention to the dynamic nature of the plastid and its interactions with the rest of the cell. Use of dual and triple fluorescent protein combinations has begun to reveal plastid interactions with mitochondria, the nucleus, the endoplasmic reticulum and F-actin and suggests integral roles of plastids in retrograde signaling, cell to cell communication as well as plant-pathogen interactions. While the rapid advances and insights achieved through fluorescent protein based research on plastids are commendable it is necessary to endorse meaningful observations but subject others to closer scrutiny. Here, in order to develop a better and more comprehensive understanding of plastids and their extensions we provide a critical appraisal of recent information that has been acquired using targeted fluorescent protein probes.
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spelling pubmed-47190812016-01-29 Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal Delfosse, Kathleen Wozny, Michael R. Jaipargas, Erica-Ashley Barton, Kiah A. Anderson, Cole Mathur, Jaideep Front Plant Sci Plant Science Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid envelope, nucleoids, plastoglobuli, and starch grains have been efficiently highlighted in living plant cells. In addition, stroma filled membrane extensions called stromules have drawn attention to the dynamic nature of the plastid and its interactions with the rest of the cell. Use of dual and triple fluorescent protein combinations has begun to reveal plastid interactions with mitochondria, the nucleus, the endoplasmic reticulum and F-actin and suggests integral roles of plastids in retrograde signaling, cell to cell communication as well as plant-pathogen interactions. While the rapid advances and insights achieved through fluorescent protein based research on plastids are commendable it is necessary to endorse meaningful observations but subject others to closer scrutiny. Here, in order to develop a better and more comprehensive understanding of plastids and their extensions we provide a critical appraisal of recent information that has been acquired using targeted fluorescent protein probes. Frontiers Media S.A. 2016-01-20 /pmc/articles/PMC4719081/ /pubmed/26834765 http://dx.doi.org/10.3389/fpls.2015.01253 Text en Copyright © 2016 Delfosse, Wozny, Jaipargas, Barton, Anderson and Mathur. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Delfosse, Kathleen
Wozny, Michael R.
Jaipargas, Erica-Ashley
Barton, Kiah A.
Anderson, Cole
Mathur, Jaideep
Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title_full Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title_fullStr Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title_full_unstemmed Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title_short Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
title_sort fluorescent protein aided insights on plastids and their extensions: a critical appraisal
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719081/
https://www.ncbi.nlm.nih.gov/pubmed/26834765
http://dx.doi.org/10.3389/fpls.2015.01253
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