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Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal
Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719081/ https://www.ncbi.nlm.nih.gov/pubmed/26834765 http://dx.doi.org/10.3389/fpls.2015.01253 |
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author | Delfosse, Kathleen Wozny, Michael R. Jaipargas, Erica-Ashley Barton, Kiah A. Anderson, Cole Mathur, Jaideep |
author_facet | Delfosse, Kathleen Wozny, Michael R. Jaipargas, Erica-Ashley Barton, Kiah A. Anderson, Cole Mathur, Jaideep |
author_sort | Delfosse, Kathleen |
collection | PubMed |
description | Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid envelope, nucleoids, plastoglobuli, and starch grains have been efficiently highlighted in living plant cells. In addition, stroma filled membrane extensions called stromules have drawn attention to the dynamic nature of the plastid and its interactions with the rest of the cell. Use of dual and triple fluorescent protein combinations has begun to reveal plastid interactions with mitochondria, the nucleus, the endoplasmic reticulum and F-actin and suggests integral roles of plastids in retrograde signaling, cell to cell communication as well as plant-pathogen interactions. While the rapid advances and insights achieved through fluorescent protein based research on plastids are commendable it is necessary to endorse meaningful observations but subject others to closer scrutiny. Here, in order to develop a better and more comprehensive understanding of plastids and their extensions we provide a critical appraisal of recent information that has been acquired using targeted fluorescent protein probes. |
format | Online Article Text |
id | pubmed-4719081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-47190812016-01-29 Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal Delfosse, Kathleen Wozny, Michael R. Jaipargas, Erica-Ashley Barton, Kiah A. Anderson, Cole Mathur, Jaideep Front Plant Sci Plant Science Multi-colored fluorescent proteins targeted to plastids have provided new insights on the dynamic behavior of these organelles and their interactions with other cytoplasmic components and compartments. Sub-plastidic components such as thylakoids, stroma, the inner and outer membranes of the plastid envelope, nucleoids, plastoglobuli, and starch grains have been efficiently highlighted in living plant cells. In addition, stroma filled membrane extensions called stromules have drawn attention to the dynamic nature of the plastid and its interactions with the rest of the cell. Use of dual and triple fluorescent protein combinations has begun to reveal plastid interactions with mitochondria, the nucleus, the endoplasmic reticulum and F-actin and suggests integral roles of plastids in retrograde signaling, cell to cell communication as well as plant-pathogen interactions. While the rapid advances and insights achieved through fluorescent protein based research on plastids are commendable it is necessary to endorse meaningful observations but subject others to closer scrutiny. Here, in order to develop a better and more comprehensive understanding of plastids and their extensions we provide a critical appraisal of recent information that has been acquired using targeted fluorescent protein probes. Frontiers Media S.A. 2016-01-20 /pmc/articles/PMC4719081/ /pubmed/26834765 http://dx.doi.org/10.3389/fpls.2015.01253 Text en Copyright © 2016 Delfosse, Wozny, Jaipargas, Barton, Anderson and Mathur. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Delfosse, Kathleen Wozny, Michael R. Jaipargas, Erica-Ashley Barton, Kiah A. Anderson, Cole Mathur, Jaideep Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title | Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title_full | Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title_fullStr | Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title_full_unstemmed | Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title_short | Fluorescent Protein Aided Insights on Plastids and their Extensions: A Critical Appraisal |
title_sort | fluorescent protein aided insights on plastids and their extensions: a critical appraisal |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719081/ https://www.ncbi.nlm.nih.gov/pubmed/26834765 http://dx.doi.org/10.3389/fpls.2015.01253 |
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