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Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes)
BACKGROUND: The genus Clarias (Clariidae, Siluriformes) contains at least 61 species naturally spread over vast regions of Asia, India and Africa. However, Clarias species have also been introduced in many different countries and represent the most widespread catfishes in the world. These fishes are...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719708/ https://www.ncbi.nlm.nih.gov/pubmed/26793275 http://dx.doi.org/10.1186/s13039-016-0215-2 |
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author | Maneechot, Nuntiya Yano, Cassia Fernanda Bertollo, Luiz Antonio Carlos Getlekha, Nuntaporn Molina, Wagner Franco Ditcharoen, Sukhonthip Tengjaroenkul, Bundit Supiwong, Weerayuth Tanomtong, Alongklod de Bello Cioffi, Marcelo |
author_facet | Maneechot, Nuntiya Yano, Cassia Fernanda Bertollo, Luiz Antonio Carlos Getlekha, Nuntaporn Molina, Wagner Franco Ditcharoen, Sukhonthip Tengjaroenkul, Bundit Supiwong, Weerayuth Tanomtong, Alongklod de Bello Cioffi, Marcelo |
author_sort | Maneechot, Nuntiya |
collection | PubMed |
description | BACKGROUND: The genus Clarias (Clariidae, Siluriformes) contains at least 61 species naturally spread over vast regions of Asia, India and Africa. However, Clarias species have also been introduced in many different countries and represent the most widespread catfishes in the world. These fishes are also known as “walking catfishes” due to their ability to move over land. A large degree of chromosomal variation has been previously found in this family, mainly using conventional cytogenetic investigations, with diploid chromosome numbers ranging between 48 and 100. In this study, we analyzed the karyotype structure and distribution of four repetitive DNA sequences (5S and 18S rDNAs and (CA)(15) and (GA)(15) microsatellites) in three Clarias species (C. batrachus, C. gariepinus, C. macrocephalus), as well as in a probable natural hybrid of the two latter species from different Thailand river basins. RESULTS: Clarias gariepinus and C. macrocephalus had 2n = 56 and 2n = 54, respectively, as well as karyotypes composed mainly by metacentric and submetacentric chromosomes. Their karyotypes differed in the number and location of 5S and 18S rDNA sites and in the degree of microsatellite accumulation. An intermediate chromosomal pattern incorporating those of the parental species was found in the probable hybrid, confirming its interspecific origin. Clarias batrachus had 2n = 104 chromosomes and its karyotype was dominated by mainly acrocentric elements, indicating that unusual multiple centric fissions were involved in its karyotype differentiation. The karyotype of this species presented an unexpected dispersion of ribosomal DNAs, possessing 54 and 12 sites of 5S and 18S rDNAs, respectively, as well as a high accumulation and differential distribution of both microsatellite repeats, representing ‘hot spots’ for chromosomal rearrangement. CONCLUSION: Both conventional and molecular cytogenetic markers were useful tools for demonstrating remarkable evolutionary dynamism and highlighting multiple chromosomal rearrangements and hybridization events correlated with the notable karyotypic diversity of these walking catfishes. |
format | Online Article Text |
id | pubmed-4719708 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47197082016-01-21 Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) Maneechot, Nuntiya Yano, Cassia Fernanda Bertollo, Luiz Antonio Carlos Getlekha, Nuntaporn Molina, Wagner Franco Ditcharoen, Sukhonthip Tengjaroenkul, Bundit Supiwong, Weerayuth Tanomtong, Alongklod de Bello Cioffi, Marcelo Mol Cytogenet Research BACKGROUND: The genus Clarias (Clariidae, Siluriformes) contains at least 61 species naturally spread over vast regions of Asia, India and Africa. However, Clarias species have also been introduced in many different countries and represent the most widespread catfishes in the world. These fishes are also known as “walking catfishes” due to their ability to move over land. A large degree of chromosomal variation has been previously found in this family, mainly using conventional cytogenetic investigations, with diploid chromosome numbers ranging between 48 and 100. In this study, we analyzed the karyotype structure and distribution of four repetitive DNA sequences (5S and 18S rDNAs and (CA)(15) and (GA)(15) microsatellites) in three Clarias species (C. batrachus, C. gariepinus, C. macrocephalus), as well as in a probable natural hybrid of the two latter species from different Thailand river basins. RESULTS: Clarias gariepinus and C. macrocephalus had 2n = 56 and 2n = 54, respectively, as well as karyotypes composed mainly by metacentric and submetacentric chromosomes. Their karyotypes differed in the number and location of 5S and 18S rDNA sites and in the degree of microsatellite accumulation. An intermediate chromosomal pattern incorporating those of the parental species was found in the probable hybrid, confirming its interspecific origin. Clarias batrachus had 2n = 104 chromosomes and its karyotype was dominated by mainly acrocentric elements, indicating that unusual multiple centric fissions were involved in its karyotype differentiation. The karyotype of this species presented an unexpected dispersion of ribosomal DNAs, possessing 54 and 12 sites of 5S and 18S rDNAs, respectively, as well as a high accumulation and differential distribution of both microsatellite repeats, representing ‘hot spots’ for chromosomal rearrangement. CONCLUSION: Both conventional and molecular cytogenetic markers were useful tools for demonstrating remarkable evolutionary dynamism and highlighting multiple chromosomal rearrangements and hybridization events correlated with the notable karyotypic diversity of these walking catfishes. BioMed Central 2016-01-20 /pmc/articles/PMC4719708/ /pubmed/26793275 http://dx.doi.org/10.1186/s13039-016-0215-2 Text en © Maneechot et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Maneechot, Nuntiya Yano, Cassia Fernanda Bertollo, Luiz Antonio Carlos Getlekha, Nuntaporn Molina, Wagner Franco Ditcharoen, Sukhonthip Tengjaroenkul, Bundit Supiwong, Weerayuth Tanomtong, Alongklod de Bello Cioffi, Marcelo Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title | Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title_full | Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title_fullStr | Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title_full_unstemmed | Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title_short | Genomic organization of repetitive DNAs highlights chromosomal evolution in the genus Clarias (Clariidae, Siluriformes) |
title_sort | genomic organization of repetitive dnas highlights chromosomal evolution in the genus clarias (clariidae, siluriformes) |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4719708/ https://www.ncbi.nlm.nih.gov/pubmed/26793275 http://dx.doi.org/10.1186/s13039-016-0215-2 |
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