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CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells

The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiat...

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Autores principales: Skelton, Rhys J.P., Brady, Bevin, Khoja, Suhail, Sahoo, Debashis, Engel, James, Arasaratnam, Deevina, Saleh, Kholoud K., Abilez, Oscar J., Zhao, Peng, Stanley, Edouard G., Elefanty, Andrew G., Kwon, Murray, Elliott, David A., Ardehali, Reza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720015/
https://www.ncbi.nlm.nih.gov/pubmed/26771355
http://dx.doi.org/10.1016/j.stemcr.2015.11.006
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author Skelton, Rhys J.P.
Brady, Bevin
Khoja, Suhail
Sahoo, Debashis
Engel, James
Arasaratnam, Deevina
Saleh, Kholoud K.
Abilez, Oscar J.
Zhao, Peng
Stanley, Edouard G.
Elefanty, Andrew G.
Kwon, Murray
Elliott, David A.
Ardehali, Reza
author_facet Skelton, Rhys J.P.
Brady, Bevin
Khoja, Suhail
Sahoo, Debashis
Engel, James
Arasaratnam, Deevina
Saleh, Kholoud K.
Abilez, Oscar J.
Zhao, Peng
Stanley, Edouard G.
Elefanty, Andrew G.
Kwon, Murray
Elliott, David A.
Ardehali, Reza
author_sort Skelton, Rhys J.P.
collection PubMed
description The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.
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spelling pubmed-47200152016-02-22 CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells Skelton, Rhys J.P. Brady, Bevin Khoja, Suhail Sahoo, Debashis Engel, James Arasaratnam, Deevina Saleh, Kholoud K. Abilez, Oscar J. Zhao, Peng Stanley, Edouard G. Elefanty, Andrew G. Kwon, Murray Elliott, David A. Ardehali, Reza Stem Cell Reports Article The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications. Elsevier 2016-01-12 /pmc/articles/PMC4720015/ /pubmed/26771355 http://dx.doi.org/10.1016/j.stemcr.2015.11.006 Text en © 2016 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Skelton, Rhys J.P.
Brady, Bevin
Khoja, Suhail
Sahoo, Debashis
Engel, James
Arasaratnam, Deevina
Saleh, Kholoud K.
Abilez, Oscar J.
Zhao, Peng
Stanley, Edouard G.
Elefanty, Andrew G.
Kwon, Murray
Elliott, David A.
Ardehali, Reza
CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title_full CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title_fullStr CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title_full_unstemmed CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title_short CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
title_sort cd13 and ror2 permit isolation of highly enriched cardiac mesoderm from differentiating human embryonic stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720015/
https://www.ncbi.nlm.nih.gov/pubmed/26771355
http://dx.doi.org/10.1016/j.stemcr.2015.11.006
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