Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy

DNA oligomers are studied at 0% and 92% relative humidity, corresponding to N < 2 and N > 20 water molecules per base pair. Two-dimensional (2D) infrared spectroscopy of DNA backbone modes between 920 and 1120 cm(−1) maps fluctuating interactions at the DNA surface. At both hydration levels, a...

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Autores principales: Guchhait, Biswajit, Liu, Yingliang, Siebert, Torsten, Elsaesser, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Crystallographic Association 2015
Materias:
SPECIAL TOPIC: THE HAMBURG CONFERENCE ON FEMTOCHEMISTRY (FEMTO12)
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720115/
https://www.ncbi.nlm.nih.gov/pubmed/26798841
http://dx.doi.org/10.1063/1.4936567
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author Guchhait, Biswajit
Liu, Yingliang
Siebert, Torsten
Elsaesser, Thomas
author_facet Guchhait, Biswajit
Liu, Yingliang
Siebert, Torsten
Elsaesser, Thomas
author_sort Guchhait, Biswajit
collection PubMed
description DNA oligomers are studied at 0% and 92% relative humidity, corresponding to N < 2 and N > 20 water molecules per base pair. Two-dimensional (2D) infrared spectroscopy of DNA backbone modes between 920 and 1120 cm(−1) maps fluctuating interactions at the DNA surface. At both hydration levels, a frequency fluctuation correlation function with a 300 fs decay and a slow decay beyond 10 ps is derived from the 2D lineshapes. The fast component reflects motions of DNA helix, counterions, and water shell. Its higher amplitude at high hydration level reveals a significant contribution of water to the fluctuating forces. The slow component reflects disorder-induced inhomogeneous broadening.
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spelling pubmed-47201152016-01-21 Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy Guchhait, Biswajit Liu, Yingliang Siebert, Torsten Elsaesser, Thomas Struct Dyn SPECIAL TOPIC: THE HAMBURG CONFERENCE ON FEMTOCHEMISTRY (FEMTO12) DNA oligomers are studied at 0% and 92% relative humidity, corresponding to N < 2 and N > 20 water molecules per base pair. Two-dimensional (2D) infrared spectroscopy of DNA backbone modes between 920 and 1120 cm(−1) maps fluctuating interactions at the DNA surface. At both hydration levels, a frequency fluctuation correlation function with a 300 fs decay and a slow decay beyond 10 ps is derived from the 2D lineshapes. The fast component reflects motions of DNA helix, counterions, and water shell. Its higher amplitude at high hydration level reveals a significant contribution of water to the fluctuating forces. The slow component reflects disorder-induced inhomogeneous broadening. American Crystallographic Association 2015-12-08 /pmc/articles/PMC4720115/ /pubmed/26798841 http://dx.doi.org/10.1063/1.4936567 Text en © 2015 Author(s). 2329-7778/2016/3(4)/043202/15 All article content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 Unported License.
spellingShingle SPECIAL TOPIC: THE HAMBURG CONFERENCE ON FEMTOCHEMISTRY (FEMTO12)
Guchhait, Biswajit
Liu, Yingliang
Siebert, Torsten
Elsaesser, Thomas
Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title_full Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title_fullStr Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title_full_unstemmed Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title_short Ultrafast vibrational dynamics of the DNA backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
title_sort ultrafast vibrational dynamics of the dna backbone at different hydration levels mapped by two-dimensional infrared spectroscopy
topic SPECIAL TOPIC: THE HAMBURG CONFERENCE ON FEMTOCHEMISTRY (FEMTO12)
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720115/
https://www.ncbi.nlm.nih.gov/pubmed/26798841
http://dx.doi.org/10.1063/1.4936567
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