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Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae
Caveolae are flask-shaped plasma membrane subdomains abundant in most cell types that participate in endocytosis. Caveola formation and functions require membrane proteins of the caveolin family, and cytoplasmic proteins of the cavin family. Cationic peptide dendrimers are non-vesicular chemical car...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720277/ https://www.ncbi.nlm.nih.gov/pubmed/26788849 http://dx.doi.org/10.1371/journal.pone.0147491 |
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author | Rewatkar, Prarthana V. Parekh, Harendra S. Parat, Marie-Odile |
author_facet | Rewatkar, Prarthana V. Parekh, Harendra S. Parat, Marie-Odile |
author_sort | Rewatkar, Prarthana V. |
collection | PubMed |
description | Caveolae are flask-shaped plasma membrane subdomains abundant in most cell types that participate in endocytosis. Caveola formation and functions require membrane proteins of the caveolin family, and cytoplasmic proteins of the cavin family. Cationic peptide dendrimers are non-vesicular chemical carriers that can transport pharmacological agents or genetic material across the plasma membrane. We prepared a panel of cationic dendrimers and investigated whether they require caveolae to enter into cells. Cell-based studies were performed using wild type or caveola-deficient i.e. caveolin-1 or PTRF gene-disrupted cells. There was a statistically significant difference in entry of cationic dendrimers between wild type and caveola-deficient cells. We further unveiled differences between dendrimers with varying charge density and head groups. Our results show, using a molecular approach, that (i) expression of caveola-forming proteins promotes cellular entry of cationic dendrimers and (ii) dendrimer structure can be modified to promote endocytosis in caveola-forming cells. |
format | Online Article Text |
id | pubmed-4720277 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-47202772016-01-30 Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae Rewatkar, Prarthana V. Parekh, Harendra S. Parat, Marie-Odile PLoS One Research Article Caveolae are flask-shaped plasma membrane subdomains abundant in most cell types that participate in endocytosis. Caveola formation and functions require membrane proteins of the caveolin family, and cytoplasmic proteins of the cavin family. Cationic peptide dendrimers are non-vesicular chemical carriers that can transport pharmacological agents or genetic material across the plasma membrane. We prepared a panel of cationic dendrimers and investigated whether they require caveolae to enter into cells. Cell-based studies were performed using wild type or caveola-deficient i.e. caveolin-1 or PTRF gene-disrupted cells. There was a statistically significant difference in entry of cationic dendrimers between wild type and caveola-deficient cells. We further unveiled differences between dendrimers with varying charge density and head groups. Our results show, using a molecular approach, that (i) expression of caveola-forming proteins promotes cellular entry of cationic dendrimers and (ii) dendrimer structure can be modified to promote endocytosis in caveola-forming cells. Public Library of Science 2016-01-20 /pmc/articles/PMC4720277/ /pubmed/26788849 http://dx.doi.org/10.1371/journal.pone.0147491 Text en © 2016 Rewatkar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rewatkar, Prarthana V. Parekh, Harendra S. Parat, Marie-Odile Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title | Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title_full | Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title_fullStr | Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title_full_unstemmed | Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title_short | Molecular Determinants of the Cellular Entry of Asymmetric Peptide Dendrimers and Role of Caveolae |
title_sort | molecular determinants of the cellular entry of asymmetric peptide dendrimers and role of caveolae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720277/ https://www.ncbi.nlm.nih.gov/pubmed/26788849 http://dx.doi.org/10.1371/journal.pone.0147491 |
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