Cargando…

Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs

Comparative studies on the distribution of archaeal versus bacterial communities associated with the surface mucus layer of corals have rarely taken place. It has therefore remained enigmatic whether mucus-associated archaeal and bacterial communities exhibit a similar specificity towards coral host...

Descripción completa

Detalles Bibliográficos
Autores principales: Frade, Pedro R., Roll, Katharina, Bergauer, Kristin, Herndl, Gerhard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720286/
https://www.ncbi.nlm.nih.gov/pubmed/26788724
http://dx.doi.org/10.1371/journal.pone.0144702
_version_ 1782411065969082368
author Frade, Pedro R.
Roll, Katharina
Bergauer, Kristin
Herndl, Gerhard J.
author_facet Frade, Pedro R.
Roll, Katharina
Bergauer, Kristin
Herndl, Gerhard J.
author_sort Frade, Pedro R.
collection PubMed
description Comparative studies on the distribution of archaeal versus bacterial communities associated with the surface mucus layer of corals have rarely taken place. It has therefore remained enigmatic whether mucus-associated archaeal and bacterial communities exhibit a similar specificity towards coral hosts and whether they vary in the same fashion over spatial gradients and between reef locations. We used microbial community profiling (terminal-restriction fragment length polymorphism, T-RFLP) and clone library sequencing of the 16S rRNA gene to compare the diversity and community structure of dominant archaeal and bacterial communities associating with the mucus of three common reef-building coral species (Porites astreoides, Siderastrea siderea and Orbicella annularis) over different spatial scales on a Caribbean fringing reef. Sampling locations included three reef sites, three reef patches within each site and two depths. Reference sediment samples and ambient water were also taken for each of the 18 sampling locations resulting in a total of 239 samples. While only 41% of the bacterial operational taxonomic units (OTUs) characterized by T-RFLP were shared between mucus and the ambient water or sediment, for archaeal OTUs this percentage was 2-fold higher (78%). About half of the mucus-associated OTUs (44% and 58% of bacterial and archaeal OTUs, respectively) were shared between the three coral species. Our multivariate statistical analysis (ANOSIM, PERMANOVA and CCA) showed that while the bacterial community composition was determined by habitat (mucus, sediment or seawater), host coral species, location and spatial distance, the archaeal community composition was solely determined by the habitat. This study highlights that mucus-associated archaeal and bacterial communities differ in their degree of community turnover over reefs and in their host-specificity.
format Online
Article
Text
id pubmed-4720286
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-47202862016-01-30 Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs Frade, Pedro R. Roll, Katharina Bergauer, Kristin Herndl, Gerhard J. PLoS One Research Article Comparative studies on the distribution of archaeal versus bacterial communities associated with the surface mucus layer of corals have rarely taken place. It has therefore remained enigmatic whether mucus-associated archaeal and bacterial communities exhibit a similar specificity towards coral hosts and whether they vary in the same fashion over spatial gradients and between reef locations. We used microbial community profiling (terminal-restriction fragment length polymorphism, T-RFLP) and clone library sequencing of the 16S rRNA gene to compare the diversity and community structure of dominant archaeal and bacterial communities associating with the mucus of three common reef-building coral species (Porites astreoides, Siderastrea siderea and Orbicella annularis) over different spatial scales on a Caribbean fringing reef. Sampling locations included three reef sites, three reef patches within each site and two depths. Reference sediment samples and ambient water were also taken for each of the 18 sampling locations resulting in a total of 239 samples. While only 41% of the bacterial operational taxonomic units (OTUs) characterized by T-RFLP were shared between mucus and the ambient water or sediment, for archaeal OTUs this percentage was 2-fold higher (78%). About half of the mucus-associated OTUs (44% and 58% of bacterial and archaeal OTUs, respectively) were shared between the three coral species. Our multivariate statistical analysis (ANOSIM, PERMANOVA and CCA) showed that while the bacterial community composition was determined by habitat (mucus, sediment or seawater), host coral species, location and spatial distance, the archaeal community composition was solely determined by the habitat. This study highlights that mucus-associated archaeal and bacterial communities differ in their degree of community turnover over reefs and in their host-specificity. Public Library of Science 2016-01-20 /pmc/articles/PMC4720286/ /pubmed/26788724 http://dx.doi.org/10.1371/journal.pone.0144702 Text en © 2016 Frade et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
spellingShingle Research Article
Frade, Pedro R.
Roll, Katharina
Bergauer, Kristin
Herndl, Gerhard J.
Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title_full Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title_fullStr Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title_full_unstemmed Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title_short Archaeal and Bacterial Communities Associated with the Surface Mucus of Caribbean Corals Differ in Their Degree of Host Specificity and Community Turnover Over Reefs
title_sort archaeal and bacterial communities associated with the surface mucus of caribbean corals differ in their degree of host specificity and community turnover over reefs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4720286/
https://www.ncbi.nlm.nih.gov/pubmed/26788724
http://dx.doi.org/10.1371/journal.pone.0144702
work_keys_str_mv AT fradepedror archaealandbacterialcommunitiesassociatedwiththesurfacemucusofcaribbeancoralsdifferintheirdegreeofhostspecificityandcommunityturnoveroverreefs
AT rollkatharina archaealandbacterialcommunitiesassociatedwiththesurfacemucusofcaribbeancoralsdifferintheirdegreeofhostspecificityandcommunityturnoveroverreefs
AT bergauerkristin archaealandbacterialcommunitiesassociatedwiththesurfacemucusofcaribbeancoralsdifferintheirdegreeofhostspecificityandcommunityturnoveroverreefs
AT herndlgerhardj archaealandbacterialcommunitiesassociatedwiththesurfacemucusofcaribbeancoralsdifferintheirdegreeofhostspecificityandcommunityturnoveroverreefs