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The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci
BACKGROUND: Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a crucial role in the transmission of schistosomiasis. The endemic area of S. japonicum is strictly consistent with the geographical distribution of O. hupensis. METHODS: A total of 24 populations...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721134/ https://www.ncbi.nlm.nih.gov/pubmed/26791938 http://dx.doi.org/10.1186/s13071-016-1321-z |
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author | Guan, Wei Li, Shi-Zhu Abe, Eniola Michael Webster, Bonnie L. Rollinson, David Zhou, Xiao-Nong |
author_facet | Guan, Wei Li, Shi-Zhu Abe, Eniola Michael Webster, Bonnie L. Rollinson, David Zhou, Xiao-Nong |
author_sort | Guan, Wei |
collection | PubMed |
description | BACKGROUND: Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a crucial role in the transmission of schistosomiasis. The endemic area of S. japonicum is strictly consistent with the geographical distribution of O. hupensis. METHODS: A total of 24 populations of O. hupensis from four ecological landscapes were selected for analysis of genetic diversity by screening eight microsatellite DNA polymorphic loci. RESULTS: The number of alleles per locus ranged from 29 to 70 with an average of 45.625 and that of effective alleles were 18.5 to 45.8 with an average of 27.4. The observed (Ho) and expected (He) heterozygosities varied from 0.331 to 0.57 and from 0.888 to 0.974, respectively. The mean of polymorphism information content (PIC) for all populations was 0.940, appearing polymorphic for all loci. For the fixation index of F-Statistics, Fit and Fst were 54.95 and 37.62 %, respectively. Variation of O. hupensis chiefly exists among individuals, accounting for 60.58 % of the total variation determined by Analysis of Molecular Variation (AMOVA). Variation among individuals within populations, among populations within groups and among groups only accounted for 26.60, 8.04 and 4.78 %, respectively. This distribution of variation suggests that genetic differences principally originate from within-populations rather than among-populations. Moreover, UPGMA cluster analysis showed that the populations spreading within middle and lower reaches of the Yangtze River (HBWH, JSYZ, JXNC, HNHS, JXJJ, AHWW, HBJL, JXDC, HNNX, JSYZJZ, ZJJH, AHNG and AHWJ) clustered together first, then gathered with the populations in the high mountains (SCMS, SCYA, SCPJ, YNEY, SCLS, YNWS and SCXC), coastal hills (FJFQ and FJFZ) and Karst landform (GXBS and GXYZ) successively. CONCLUSION: This study provides novel insight into the theoretical source of genetic differentiation of Oncomelania hupensis in mainland China, which is critical for the epidemiological investigation and surveillance of S. japonicum. |
format | Online Article Text |
id | pubmed-4721134 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-47211342016-01-22 The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci Guan, Wei Li, Shi-Zhu Abe, Eniola Michael Webster, Bonnie L. Rollinson, David Zhou, Xiao-Nong Parasit Vectors Research BACKGROUND: Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum, which plays a crucial role in the transmission of schistosomiasis. The endemic area of S. japonicum is strictly consistent with the geographical distribution of O. hupensis. METHODS: A total of 24 populations of O. hupensis from four ecological landscapes were selected for analysis of genetic diversity by screening eight microsatellite DNA polymorphic loci. RESULTS: The number of alleles per locus ranged from 29 to 70 with an average of 45.625 and that of effective alleles were 18.5 to 45.8 with an average of 27.4. The observed (Ho) and expected (He) heterozygosities varied from 0.331 to 0.57 and from 0.888 to 0.974, respectively. The mean of polymorphism information content (PIC) for all populations was 0.940, appearing polymorphic for all loci. For the fixation index of F-Statistics, Fit and Fst were 54.95 and 37.62 %, respectively. Variation of O. hupensis chiefly exists among individuals, accounting for 60.58 % of the total variation determined by Analysis of Molecular Variation (AMOVA). Variation among individuals within populations, among populations within groups and among groups only accounted for 26.60, 8.04 and 4.78 %, respectively. This distribution of variation suggests that genetic differences principally originate from within-populations rather than among-populations. Moreover, UPGMA cluster analysis showed that the populations spreading within middle and lower reaches of the Yangtze River (HBWH, JSYZ, JXNC, HNHS, JXJJ, AHWW, HBJL, JXDC, HNNX, JSYZJZ, ZJJH, AHNG and AHWJ) clustered together first, then gathered with the populations in the high mountains (SCMS, SCYA, SCPJ, YNEY, SCLS, YNWS and SCXC), coastal hills (FJFQ and FJFZ) and Karst landform (GXBS and GXYZ) successively. CONCLUSION: This study provides novel insight into the theoretical source of genetic differentiation of Oncomelania hupensis in mainland China, which is critical for the epidemiological investigation and surveillance of S. japonicum. BioMed Central 2016-01-20 /pmc/articles/PMC4721134/ /pubmed/26791938 http://dx.doi.org/10.1186/s13071-016-1321-z Text en © Guan et al. 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Guan, Wei Li, Shi-Zhu Abe, Eniola Michael Webster, Bonnie L. Rollinson, David Zhou, Xiao-Nong The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title | The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title_full | The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title_fullStr | The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title_full_unstemmed | The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title_short | The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci |
title_sort | genetic diversity and geographical separation study of oncomelania hupensis populations in mainland china using microsatellite loci |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721134/ https://www.ncbi.nlm.nih.gov/pubmed/26791938 http://dx.doi.org/10.1186/s13071-016-1321-z |
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