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Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves
CONTEXT: Centella asiatica (CA) leaves extract has been shown therapeutic potential. However, safety information is lacking. AIMS: To evaluate acute oral toxicity (AOT), sub-chronic toxicity, and mutagenic potential of standardized extract of CA (L.) Urban leaves (INDCA). MATERIALS AND METHODS: For...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721154/ https://www.ncbi.nlm.nih.gov/pubmed/26862255 http://dx.doi.org/10.4103/0971-6580.172251 |
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author | Deshpande, Pallavi O. Mohan, Vishwaraman Thakurdesai, Prasad |
author_facet | Deshpande, Pallavi O. Mohan, Vishwaraman Thakurdesai, Prasad |
author_sort | Deshpande, Pallavi O. |
collection | PubMed |
description | CONTEXT: Centella asiatica (CA) leaves extract has been shown therapeutic potential. However, safety information is lacking. AIMS: To evaluate acute oral toxicity (AOT), sub-chronic toxicity, and mutagenic potential of standardized extract of CA (L.) Urban leaves (INDCA). MATERIALS AND METHODS: For the acute toxicity study, INDCA was orally administered to Sprague-Dawley rats at a dose range of 0–2000 mg/kg. For the repeated dose toxicity study, the rats of either sex were orally administered with INDCA at the doses of 250, 500, and 1000 mg/kg/day for a period of 90 days. The effects on body weight, food and water consumption, organ weight, hematology, clinical chemistry as well as histology were studied. The mutagenic potential of INDCA was tested using reverse mutation assay (Ames test). STATISTICAL ANALYSIS USED: Data of each parameter were analyzed by one-way ANOVA followed by Dunnett's test to compare the difference between treated groups. RESULTS: The administration of INDCA did not produce mortality or significant changes in the clinical signs included but not limited to changes in the skin and fur, eyes and mucous membranes, and also respiratory, circulatory, autonomic and central nervous systems, somatomotor activity, and behavior pattern. The appearance, progress, and disappearance of these signs were recorded. The lethal dose and no observable adverse effect level of INDCA were 2000 mg/kg and 1000 mg/kg, respectively. There were no significant differences in the organ weights, hematological parameters, clinical chemistry values, or gross and microscopic appearance of the organs from the treatment groups as compared to the control group. It was found to be nonmutagenic in reverse mutation assay. CONCLUSIONS: INDCA was found safe in AOT, sub-chronic toxicity, and mutagenicity studies when tested in rats. |
format | Online Article Text |
id | pubmed-4721154 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-47211542016-02-09 Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves Deshpande, Pallavi O. Mohan, Vishwaraman Thakurdesai, Prasad Toxicol Int Original Article CONTEXT: Centella asiatica (CA) leaves extract has been shown therapeutic potential. However, safety information is lacking. AIMS: To evaluate acute oral toxicity (AOT), sub-chronic toxicity, and mutagenic potential of standardized extract of CA (L.) Urban leaves (INDCA). MATERIALS AND METHODS: For the acute toxicity study, INDCA was orally administered to Sprague-Dawley rats at a dose range of 0–2000 mg/kg. For the repeated dose toxicity study, the rats of either sex were orally administered with INDCA at the doses of 250, 500, and 1000 mg/kg/day for a period of 90 days. The effects on body weight, food and water consumption, organ weight, hematology, clinical chemistry as well as histology were studied. The mutagenic potential of INDCA was tested using reverse mutation assay (Ames test). STATISTICAL ANALYSIS USED: Data of each parameter were analyzed by one-way ANOVA followed by Dunnett's test to compare the difference between treated groups. RESULTS: The administration of INDCA did not produce mortality or significant changes in the clinical signs included but not limited to changes in the skin and fur, eyes and mucous membranes, and also respiratory, circulatory, autonomic and central nervous systems, somatomotor activity, and behavior pattern. The appearance, progress, and disappearance of these signs were recorded. The lethal dose and no observable adverse effect level of INDCA were 2000 mg/kg and 1000 mg/kg, respectively. There were no significant differences in the organ weights, hematological parameters, clinical chemistry values, or gross and microscopic appearance of the organs from the treatment groups as compared to the control group. It was found to be nonmutagenic in reverse mutation assay. CONCLUSIONS: INDCA was found safe in AOT, sub-chronic toxicity, and mutagenicity studies when tested in rats. Medknow Publications & Media Pvt Ltd 2015 /pmc/articles/PMC4721154/ /pubmed/26862255 http://dx.doi.org/10.4103/0971-6580.172251 Text en Copyright: © Toxicology International http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution NonCommercial ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Deshpande, Pallavi O. Mohan, Vishwaraman Thakurdesai, Prasad Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title | Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title_full | Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title_fullStr | Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title_full_unstemmed | Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title_short | Preclinical Safety Assessment of Standardized Extract of Centella asiatica (L.) Urban Leaves |
title_sort | preclinical safety assessment of standardized extract of centella asiatica (l.) urban leaves |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721154/ https://www.ncbi.nlm.nih.gov/pubmed/26862255 http://dx.doi.org/10.4103/0971-6580.172251 |
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