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Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos)
The duck is one of the most economically important waterfowl as a source of meat, eggs, and feathers. Characterizing the genetic variation in duck species is an important step toward linking genes or genomic regions with phenotypes. Human-driven selection during duck domestication and subsequent bre...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Libertas Academica
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721680/ https://www.ncbi.nlm.nih.gov/pubmed/26819540 http://dx.doi.org/10.4137/EBO.S21545 |
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author | Lin, Ruiyi Du, Xiaoyong Peng, Sixue Yang, Liubin Ma, Yunlong Gong, Yanzhang Li, Shijun |
author_facet | Lin, Ruiyi Du, Xiaoyong Peng, Sixue Yang, Liubin Ma, Yunlong Gong, Yanzhang Li, Shijun |
author_sort | Lin, Ruiyi |
collection | PubMed |
description | The duck is one of the most economically important waterfowl as a source of meat, eggs, and feathers. Characterizing the genetic variation in duck species is an important step toward linking genes or genomic regions with phenotypes. Human-driven selection during duck domestication and subsequent breed formation has likely left detectable signatures in duck genome. In this study, we employed a panel of >1.4 million single-nucleotide polymorphisms (SNPs) identified from the RNA sequencing (RNA-seq) data of 15 duck individuals. The density of the resulting SNPs is significantly positively correlated with the density of genes across the duck genome, which demonstrates that the usage of the RNA-seq data allowed us to enrich variant functional categories, such as coding exons, untranslated regions (UTRs), introns, and downstream/upstream. We performed a complete scan of selection signatures in the ducks using the composite likelihood ratio (CLR) and found 76 candidate regions of selection, many of which harbor genes related to phenotypes relevant to the function of the digestive system and fat metabolism, including TCF7L2, EIF2AK3, ELOVL2, and fatty acid-binding protein family. This study illustrates the potential of population genetic approaches for identifying genomic regions affecting domestication-related phenotypes and further helps to increase the known genetic information about this economically important animal. |
format | Online Article Text |
id | pubmed-4721680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-47216802016-01-27 Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) Lin, Ruiyi Du, Xiaoyong Peng, Sixue Yang, Liubin Ma, Yunlong Gong, Yanzhang Li, Shijun Evol Bioinform Online Original Research The duck is one of the most economically important waterfowl as a source of meat, eggs, and feathers. Characterizing the genetic variation in duck species is an important step toward linking genes or genomic regions with phenotypes. Human-driven selection during duck domestication and subsequent breed formation has likely left detectable signatures in duck genome. In this study, we employed a panel of >1.4 million single-nucleotide polymorphisms (SNPs) identified from the RNA sequencing (RNA-seq) data of 15 duck individuals. The density of the resulting SNPs is significantly positively correlated with the density of genes across the duck genome, which demonstrates that the usage of the RNA-seq data allowed us to enrich variant functional categories, such as coding exons, untranslated regions (UTRs), introns, and downstream/upstream. We performed a complete scan of selection signatures in the ducks using the composite likelihood ratio (CLR) and found 76 candidate regions of selection, many of which harbor genes related to phenotypes relevant to the function of the digestive system and fat metabolism, including TCF7L2, EIF2AK3, ELOVL2, and fatty acid-binding protein family. This study illustrates the potential of population genetic approaches for identifying genomic regions affecting domestication-related phenotypes and further helps to increase the known genetic information about this economically important animal. Libertas Academica 2015-12-01 /pmc/articles/PMC4721680/ /pubmed/26819540 http://dx.doi.org/10.4137/EBO.S21545 Text en © 2015 the author(s), publisher and licensee Libertas Academica Ltd. This is an open-access article distributed under the terms of the Creative Commons CC-BY-NC 3.0 License. |
spellingShingle | Original Research Lin, Ruiyi Du, Xiaoyong Peng, Sixue Yang, Liubin Ma, Yunlong Gong, Yanzhang Li, Shijun Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title | Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title_full | Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title_fullStr | Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title_full_unstemmed | Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title_short | Discovering All Transcriptome Single-Nucleotide Polymorphisms and Scanning for Selection Signatures in Ducks (Anas platyrhynchos) |
title_sort | discovering all transcriptome single-nucleotide polymorphisms and scanning for selection signatures in ducks (anas platyrhynchos) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4721680/ https://www.ncbi.nlm.nih.gov/pubmed/26819540 http://dx.doi.org/10.4137/EBO.S21545 |
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