Cargando…
A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility
The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality an...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4722510/ https://www.ncbi.nlm.nih.gov/pubmed/26925375 http://dx.doi.org/10.1016/j.mgene.2015.07.004 |
_version_ | 1782411368898494464 |
---|---|
author | Kumar, Sushil Singh, Umesh Deb, Rajib Tyagi, Shrikant Mandal, D.K. Kumar, Mahesh Sengar, G. Sharma, Sheetal Singh, Rani Singh, Rupali |
author_facet | Kumar, Sushil Singh, Umesh Deb, Rajib Tyagi, Shrikant Mandal, D.K. Kumar, Mahesh Sengar, G. Sharma, Sheetal Singh, Rani Singh, Rupali |
author_sort | Kumar, Sushil |
collection | PubMed |
description | The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nucleotide polymorphism (g.358A > T) in intron 6 was significantly associated with sperm concentration (P < 0.05) and motility percentage (P < 0.01). mRNA was extracted from good (progressive motility > 50%) and motility impaired (progressive motility < 50%) bull semen. The mRNA expression and seminal plasma protein concentration of CD9 was significantly (P < 0.05) higher among good quality bull semen than motility impaired ones. Our results thus may indicate that, mutation in the intronic region may be responsible for the instability of RNA and the subsequent functional protein expression. |
format | Online Article Text |
id | pubmed-4722510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-47225102016-02-26 A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility Kumar, Sushil Singh, Umesh Deb, Rajib Tyagi, Shrikant Mandal, D.K. Kumar, Mahesh Sengar, G. Sharma, Sheetal Singh, Rani Singh, Rupali Meta Gene Article The surface expression of CD9 (cluster-of-differentiation antigen-9) in sperms of certain mammalian species has been attributed to its fusion with the egg and thereby dictating the fertility of species. In the present study, we investigated the association of CD9 with crossbred bull sperm quality and quantity trait was analyzed using a total of 96 Frieswal (HF × Sahiwal) crossbred. A single nucleotide polymorphism (g.358A > T) in intron 6 was significantly associated with sperm concentration (P < 0.05) and motility percentage (P < 0.01). mRNA was extracted from good (progressive motility > 50%) and motility impaired (progressive motility < 50%) bull semen. The mRNA expression and seminal plasma protein concentration of CD9 was significantly (P < 0.05) higher among good quality bull semen than motility impaired ones. Our results thus may indicate that, mutation in the intronic region may be responsible for the instability of RNA and the subsequent functional protein expression. Elsevier 2015-07-24 /pmc/articles/PMC4722510/ /pubmed/26925375 http://dx.doi.org/10.1016/j.mgene.2015.07.004 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Kumar, Sushil Singh, Umesh Deb, Rajib Tyagi, Shrikant Mandal, D.K. Kumar, Mahesh Sengar, G. Sharma, Sheetal Singh, Rani Singh, Rupali A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title | A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title_full | A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title_fullStr | A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title_full_unstemmed | A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title_short | A SNP (g.358A > T) at intronic region of CD9 molecule of crossbred bulls may associate with spermatozoal motility |
title_sort | snp (g.358a > t) at intronic region of cd9 molecule of crossbred bulls may associate with spermatozoal motility |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4722510/ https://www.ncbi.nlm.nih.gov/pubmed/26925375 http://dx.doi.org/10.1016/j.mgene.2015.07.004 |
work_keys_str_mv | AT kumarsushil asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhumesh asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT debrajib asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT tyagishrikant asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT mandaldk asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT kumarmahesh asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT sengarg asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT sharmasheetal asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhrani asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhrupali asnpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT kumarsushil snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhumesh snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT debrajib snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT tyagishrikant snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT mandaldk snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT kumarmahesh snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT sengarg snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT sharmasheetal snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhrani snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility AT singhrupali snpg358atatintronicregionofcd9moleculeofcrossbredbullsmayassociatewithspermatozoalmotility |