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A New Generation of FRET Sensors for Robust Measurement of Gα(i1), Gα(i2) and Gα(i3) Activation Kinetics in Single Cells

G-protein coupled receptors (GPCRs) can activate a heterotrimeric G-protein complex with subsecond kinetics. Genetically encoded biosensors based on Förster resonance energy transfer (FRET) are ideally suited for the study of such fast signaling events in single living cells. Here we report on the c...

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Detalles Bibliográficos
Autores principales:	van Unen, Jakobus, Stumpf, Anette D., Schmid, Benedikt, Reinhard, Nathalie R., Hordijk, Peter L., Hoffmann, Carsten, Gadella, Theodorus W. J., Goedhart, Joachim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2016
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4723041/ https://www.ncbi.nlm.nih.gov/pubmed/26799488 http://dx.doi.org/10.1371/journal.pone.0146789

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